LOCUS       sequence-65792-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Dunlop MJ, Dossani ZY, Szmidt HL, Chu HC, Lee TS, Keasling JD, Hadi 
            MZ, Mukhopadhyay A
  TITLE     Engineering microbial biofuel tolerance and export using efflux 
            pumps.
  JOURNAL   Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
  PUBMED    21556065
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2011-05-10"; volume: "7:487. doi"; pages: " 
            10.1038/msb.2011.21"
COMMENT     Sequence Label: pBbA5k-EPL123
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..23)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
ORIGIN
        1 tggtgatggt gatgatgatg atgatcgaca gtatggctgt gctcgatatc ttcattcttg
       61 cggctaaagc ggcggcgaac caccacaaag aataccggaa cgaagaagat tgccagtacc
      121 gttgcggtca ccatcccgcc cattacaccg gtacctactg cgttctgcgc gccggaacca
      181 gcaccagtac tgataaccag cggcataacg ccgaggataa acgccagcga ggtcatcagg
      241 atcggacgta aacgcatccg caccgcatca agcgtcgctt caatcagacc tttaccttct
      301 ttatccatca agtctttggc gaattcgacg ataaggatcg cgttcttcgc cgacaaccca
      361 atggttgtga gcaggcctac ctggaagtaa acgtcattgg tcaggccacg gaaggtggca
      421 gccagcaacg caccgataac ccccagcgga acgaccagca taacggagaa cggaatcgac
      481 cagctctcgt acagcgccgc cagacacagg aacacgacaa tcaacgaaat cgcgtacagt
      541 gaaggtgcct ggttgccgga gagacgttcc tgataggaca tccccgtcca gtcatagcca
      601 acaccggtag gcagtttgct cgccagttgt tccatcagct ccattgcttc accggtactt
      661 ttacccggtg ccgcctggcc taagatttcc atggatggca ggccgttgta acgttccaga
      721 cgcggcgaac cgtactccca acgagaagag gagaacgccg agaatggcac catctgacca
      781 tcagcagcac gaacatacca gtcgccgata tcatccggca gcatacggta tttcgcttct
      841 gacatgacat aaactttctt cacacgaccg cggtcgataa agtcgttc
//
