LOCUS       sequence-65799-e        1254 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Red intensiometric genetically encoded Ca2+-indicators for optical 
            imaging 1.2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Wu J, Liu L, Matsuda T, Zhao Y, Rebane A, Drobizhev M, Chang YF, 
            Araki S, Arai Y, March K, Hughes TE, Sagou K, Miyata T, Nagai T, Li 
            WH, Campbell RE
  TITLE     Improved Orange and Red Ca Indicators and Photophysical 
            Considerations for Optogenetic Applications.
  JOURNAL   ACS Chem Neurosci. 2013 Mar 19.
  PUBMED    23452507
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Neurosci. 2013 Mar 19."
COMMENT     Sequence Label: CMV-R-GECO1.2
FEATURES             Location/Qualifiers
     source          1..1254
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..72
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     768..787
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggtcgact catcacgtcg taagtggaat aaggcaggtc acgcagtcag agctataggt
       61 cggctgagct cacccgtggt ttccgagcgg atgtaccccg aggacggcgc cctgaagagc
      121 gagatcaaga aggggctgag gctgaaggac ggcggccact acgccgccga ggtcaagacc
      181 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acatcgtcga catcaagttg
      241 gacatcgtgt cccacaacga ggactacacc atcgtggaac agtgcgaacg cgccgagggc
      301 cgccactcca ccggcggcat ggacgagctg tacaagggag gtacaggcgg gagtctggtg
      361 agcaagggcg aggaggataa cagggccatc gtcaaggagt tcatgcgctt caagttgcac
      421 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      481 tacgaggcct ttcagaccgc taagctgaag gtgaccaagg gtggccccct gcccttcgca
      541 tgggacatcc tgtcccctca gttgatgtac ggctccaagg cctacattaa gcacccagcc
      601 gacatccccg actacttcaa gctgtccttc cccgagggct tcaggtggga gcgcgtgatg
      661 aacttcgagg acggcggcat tattcacgtt agccaggaca cctccctgca ggacggcgta
      721 ttcatctaca aggtgaagct gcgcggcacc aacttccccc ccgacggccc cgtaatgcag
      781 aagaagacca tgggctggga ggctacgcgt gaccaactga ctgaagagca gatcgcagaa
      841 tttaaagagg ctttctccct atttgacaag gacggggatg ggacgatgac aaccaaggag
      901 ctggggacgg tgatgcggtc tctggggcag aaccccacag aagcagagct gcaggacatg
      961 atcaatgaag tagatgccga cggtgacggc acattcgact tccctgagtt cctgacgatg
     1021 atggcaagaa aaatgaatga cacagacagt gaagaggaaa ttagagaagc gttccgcgtg
     1081 tttgataagg acggcaatgg ctacatcggc gcagcagagc ttcgccacgt gatgacagac
     1141 cttggagaga agataacaga tgaggaggtt gatgaaatga tcagggtagc agacatcgat
     1201 ggggatggtc aggtaaacta tgaagagttt gtacaaatga tgacagcgaa gtag
//
