LOCUS       sequence-65800-e        1458 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Carmine intensiometric genetically encoded Ca2+-indicators for 
            optical imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Wu J, Liu L, Matsuda T, Zhao Y, Rebane A, Drobizhev M, Chang YF, 
            Araki S, Arai Y, March K, Hughes TE, Sagou K, Miyata T, Nagai T, Li 
            WH, Campbell RE
  TITLE     Improved Orange and Red Ca Indicators and Photophysical 
            Considerations for Optogenetic Applications.
  JOURNAL   ACS Chem Neurosci. 2013 Mar 19.
  PUBMED    23452507
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Neurosci. 2013 Mar 19."
COMMENT     Sequence Label: CMV-mito-CAR-GECO1
FEATURES             Location/Qualifiers
     source          1..1458
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..75
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             97..171
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             220..276
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     972..991
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1015..1458
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTFDFPEFLTMMARKMNDTDSEEEIREAFRVFDKDGNGYIGAAEL
                     RHVMTDLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK"
ORIGIN
        1 atgtctgttc tgactcctct gctgctccgg ggtctcacag gttccgcaag aagactcccc
       61 gtgcctaggg ccaaaattca ttcactgggg gaccccatga gcgtgctcac cccactcctg
      121 ctgcgggggc tgaccggcag cgctaggcgg ctgccagtcc ccagggccaa gatccacagt
      181 ctcggcgatc ccaaggatcc aaccatggtc gactcatcac gtcgtaagtg gaataaggca
      241 ggtcacgcag ttagagctat aggtcggctg agctcacccg tggtttccga gcggatgtac
      301 cccgaggacg gcgccctgaa gagcgagatc aagaaggggc tgaggctgaa ggacggcggc
      361 cactacgccg ccgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
      421 gcctacatcg tcgacatcaa gttggacatc gtgtcccaca acgaggacta caccatcgtg
      481 gaacagtgcg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
      541 ggaggtacag gcgggagtct ggtgagcaag ggcgtggagg ataacatggc catcgtcaag
      601 gagttcatgc gcttcaagac gcacatagag ggctccgtga acggccacga gttcgagatc
      661 gagggcgagg gcgagggccg cccctacgag gcctttcaga ccgctaagct gaaggtgacc
      721 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagattat gtacggctcc
      781 aaggcctaca ttaagcaccc agccgacatc cccgactact tcaagctgtc cttccccgag
      841 ggcttcaggt gggagcgcgt gatgaacttc gaggacggcg gcattattca cgttaaccag
      901 gactcctccc tgcaggacgg cgtattcatc tacaaggtga agctgcgcgg caccaacttc
      961 ccccccgacg gccccgtaat gcagaagaag accatgggct gggagcctac gcgtgaccaa
     1021 ctgactgaag agcagatcgc agaatttaaa gaggctttct ccctatttga caaggacggg
     1081 gatgggacga taacaaccaa ggagctgggg acggtgatgc ggtctctggg gcagaacccc
     1141 acagaagcag agctgcagga catgatcaat gaagtagatg ccgacggtga cggcacattc
     1201 gacttccctg agttcctgac gatgatggca agaaaaatga atgacacaga cagtgaagag
     1261 gaaattagag aagcgttccg cgtgtttgat aaggacggca atggctacat cggcgcagca
     1321 gagcttcgcc acgtgatgac agaccttgga gagaagttaa cagatgagga ggttgatgaa
     1381 atgatcaggg tagcagacat cgatggggat ggtcaggtaa actacgaaga gtttgtacaa
     1441 atgatgacag cgaagtaa
//
