LOCUS       sequence-65819-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The codon-optimized Cas9 nuclease under the control of the 
            Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is 
            low copy number..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Gratz SJ, Cummings AM, Nguyen JN, Hamm DC, Donohue LK, Harrison MM, 
            Wildonger J, O'Connor-Giles KM
  TITLE     Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 
            nuclease.
  JOURNAL   Genetics. 2013 May 24.
  PUBMED    23709638
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2013 May 24."
COMMENT     Sequence Label: pHsp70-Cas9
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     147..168
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             219..284
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             291..311
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 attcaattca aacaagcaaa gtgaacacgt cgctaagcga aagctaagca aataaacaag
       61 cgcagctgaa caagctaaac aatctgcagt aaagtgcaag ttaaagtgaa tcaattaaaa
      121 gtaaccagca accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa gtaattattg
      181 aatacaagaa gagaactctg ggggatctga tcgatatgga ctataaggac cacgacggag
      241 actacaagga tcatgatatt gattacaaag acgatgacga taagatggcc ccaaagaaga
      301 agcggaaggt cggtatccac ggagtcccag cagccgacaa gaagtacagc atcggcctgg
      361 acatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca
      421 agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag
      481 ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa
      541 gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga
      601 tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg
      661 ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg
      721 agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg
      781 acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga
      841 tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc
      901 agac
//
