LOCUS       sequence-65820-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Jackson WT, Giddings TH, Taylor MP, Mulinyawe S, Rabinovitch M, 
            Kopito RR, Kirkegaard K
  TITLE     Subversion of cellular autophagosomal machinery by RNA viruses.
  JOURNAL   PLoS Biol. 2005 May . 3(5):e156.
  PUBMED    15884975
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Biol. 
            2005 May . 3(5):e156."
COMMENT     Sequence Label: EGFP-LC3
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(187..561)
                     /codon_start=1
                     /gene="MAP1LC3B"
                     /product="human LC3 protein"
                     /label=LC3B
                     /note="Atg8 family member found on autophagosomes"
                     /translation="MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLP
                     VLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKD
                     EDGFLYMVYASQETFGMKLSV"
     primer_bind     complement(637..658)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     986..1005
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
       61 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
      121 attatgatca gttatctaga tccggtggat cccgggcccg cggtaccgtc gactgcagaa
      181 ttcttacact gacaatttca tcccgaacgt ctcctgggag gcatagacca tgtacaggaa
      241 tccatcttca tctttctcac tctcatacac ctctgagatt ggtgtggaga cgctgaccat
      301 gctgtgtccg ttcaccaaca ggaagaaggc ctgattagca ttgagctgta agcgccttct
      361 aattatcttg atgagctcac tcatgttgac atggtcaggt acaaggaact ttgttttatc
      421 cagaacagga agctgcttct cacccttgta tcgttctatt atcaccggga ttttggttgg
      481 atgctgctct cgaataagtc ggacatcttc tactctttgt tcgaaggtgc ggcgctgctt
      541 gaaggtcttc tccgacggca tgggaattcg aagcttgagc tcgagatctg agtacttgta
      601 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
      661 atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt agtggttgtc
      721 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
      781 cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg
      841 cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag
      901 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
      961 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
     1021 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtc
//
