LOCUS       sequence-65833-e         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Gallo CM, Munro E, Rasoloson D, Merritt C, Seydoux G
  TITLE     Processing bodies and germ granules are distinct RNA granules that 
            interact in C. elegans embryos.
  JOURNAL   Dev Biol. 2008 Nov 1. 323(1):76-87.
  PUBMED    18692039
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2008 Nov 1. 323(1):76-87."
COMMENT     Sequence Label: pID2.02
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    48..172
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(213..518)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(315..334)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 aatatacaaa acaaaatatg gaaaatacgg caaaattagg atcatcaacc actttgtaca
       61 agaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc
      121 ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta tggtcgacct
      181 gcagactggc tgtgtataag ggagcctgac atttatattc cccagaacat caggttaatg
      241 gcgtttttga tgtcattttc gcggtggctg agatcagcca cttcttcccc gataacggag
      301 accggcacac tggccatatc ggtggtcatc atgcgccagc tttcatcccc gatatgcacc
      361 accgggtaaa gttcacggga gactttatct gacagcagac gtgcactggc cagggggatc
      421 accatccgtc gcccgggcgt gtcaataata tcactctgta catccacaaa cagacgataa
      481 cggctctctc ttttataggt gtaaacctta aactgcattt caccagcccc tgttctcgtc
      541 agcaaaagag ccgttcattt caataaaccg ggcgacctca gccatccctt cctgattttc
      601 cgctttccag cgttcggcac gcagacgacg ggcttcattc tgcatggttg tgcttaccag
      661 accggagata ttgacatcat atatgccttg agcaactgat agctgtcgct gtcaactgtc
      721 actgtaatac gctgcttcat agcatacctc tttttgacat acttcgggta tacatatcag
      781 tatatattct tataccgcaa aaatcagcgc gcaaatacgc atactgttat ctggctttta
      841 gtaagccgga tccagatctt tacgccccgc cctgccactc atcgcagtac tgttgtaatt
      901 cattaagcat tctgccgaca tggaagccat cac
//
