LOCUS       sequence-65841-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Dunlop MJ, Dossani ZY, Szmidt HL, Chu HC, Lee TS, Keasling JD, Hadi 
            MZ, Mukhopadhyay A
  TITLE     Engineering microbial biofuel tolerance and export using efflux 
            pumps.
  JOURNAL   Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
  PUBMED    21556065
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2011-05-10"; volume: "7:487. doi"; pages: " 
            10.1038/msb.2011.21"
COMMENT     Sequence Label: pBbA5k-EPL64
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtggtgatgg tgatgatggg cgggctgtgt ctccagtcgt cgcccgctgc catccgccct
       61 gatcctgaac cagatcgagt acatggccgg cagaaacacc agggtcagca ccgtacccgc
      121 aaaggtgccg ccgatcagcg tgtaggccag cgtgccccag aacaccgaat gggtgagcgg
      181 gataaaggcc agaatggcgg ccagcgccgt cagtatcacg ggacgcgcac gttgtacggt
      241 agcctcgacc accgcttgaa acgggtcgag ccccgcctgc tcgttgtggt gtatctggcc
      301 aatcagaatc agcgtattgc gcatcagaat gccggacagt gcgatcagcc cgaccagtgc
      361 attgatgccg aatggctgct gaaagagaat caacgtaggc accacaccga tcagccccag
      421 cggactggtc aggaagacca tgaccatggc ggatatcgaa cgcacctgaa gaatcagaat
      481 gatcagcgtg accgccagca tgatcggaaa caatggcaac atcgctgtca tggccttgcc
      541 ggactcctcg attgaacctg cctgctcgat ccggtaaccg ctgggcagct tgtcgataat
      601 cggctgtaac tgtcgggtta tggctgtcga cacatccggt ggttgcaggc cgtcggctat
      661 gttgccccgc acagtgatgg tcggcgtgcg gtcgcgccgt cgcatcaccg gttcctccat
      721 acgcacatca acggtatcaa tctgcgacag cggaacacgt tgcccgtcga cgccggtgag
      781 ggtgaagtcc attatccttg ccggatcaag tcgggtgtcg ccagcggagc gtgcaaccac
      841 ctgcacggtg cgaatatcct cgcgcact
//
