LOCUS       sequence-65843-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Dunlop MJ, Dossani ZY, Szmidt HL, Chu HC, Lee TS, Keasling JD, Hadi 
            MZ, Mukhopadhyay A
  TITLE     Engineering microbial biofuel tolerance and export using efflux 
            pumps.
  JOURNAL   Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
  PUBMED    21556065
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2011-05-10"; volume: "7:487. doi"; pages: " 
            10.1038/msb.2011.21"
COMMENT     Sequence Label: pBbA5k-EPL65
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtggtgatgg tgatgatgct tgtgaagggc cagcgcgggt gcggcgggcg ccgggtggcg
       61 tttatgaaac agccgctcca gttgcaggta aatgaccggc gtagtgaaca gggtcagcat
      121 ctggctcacc agcaggccac cgaccactgc gatacccaac ggttgacgca gttcggcacc
      181 gaccccgaaa cccagcatca gcggcagtgc gccgagcagg gcggcaaggg tggtcatgat
      241 aatcggtcgg aagcgggtaa tgcaggcctc ataaatggcc tcctgtgacg acaggccctg
      301 ctcacgctgc gcttgcaggg caaagtccac gagcagaatg ccgttcttct tgacgatgcc
      361 gatcagcagc acgatgccga tcagggccat gatcgagaag tcctgcccca tcatccatag
      421 cagcaacagc gcgccgatac ctgccgaggg gagggtggag ataatggtca gcgggtgcac
      481 gaagctttca taaagcacac ccagaatgat gtacaccgcc accagcgcgg cgagaatcag
      541 ccacggctgg ttggccagcg aactctggaa tgcctgcgcc gcaccctgaa aactgccgat
      601 gatcgaggca ggcatgccga tctctttctt ggcctgatcg agcatgctta ccgcgtcgcc
      661 cagtgcgact ccggacgcca ggttgaatga caggttggca gccgggaaca taccgtcgtg
      721 gctgatcgac agcgggccca tgcgcggcgc actgactttg gccagtgccg acagcggcac
      781 catttcattg gtcagcggtg agcgcaggta gaagtaagcc aggctttcgg ccttgccacg
      841 ctgctgggca tccagttc
//
