LOCUS       sequence-65847-e        9622 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Same as pdCas9, but with the w-dCas9 fusion (w is fused at the 
            N-terminal end of dCas9).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9622)
  AUTHORS   Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA
  TITLE     Programmable repression and activation of bacterial gene expression 
            using an engineered CRISPR-Cas system.
  JOURNAL   Nucleic Acids Res. 2013 Jun 12.
  PUBMED    23761437
REFERENCE   2  (bases 1 to 9622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Jun 12."
COMMENT     Sequence Label: pWJ68
FEATURES             Location/Qualifiers
     source          1..9622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     134..153
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(220..322)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(714..731)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(848..1393)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(928..947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1505..1510,1511..1527,1528..1533)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     misc_RNA        complement(1851..1929)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     CDS             2245..2517
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     CDS             join(2524..2547,2548..2550,2551..5037,5038..5040,
                     5041..6627)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    6648..6779
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
     repeat_region   6780..6815
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   6846..6881
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     primer_bind     complement(7310..7329)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(join(9182..9622,1..219))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
       61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
      121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
      181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
      241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
      301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
      361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
      421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
      481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
      541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
      601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
      661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
      721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
      781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
      841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
      901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
      961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca
     1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
     1501 atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct
     1561 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg
     1621 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg
     1681 attacgaaat catcctgtgg agcttagtag gtttagcaag atggcagcgc ctaaatgtag
     1741 aatgataaaa ggattaagag attaatttcc ctaaaaatga taaaacaagc gttttgaaag
     1801 cgcttgtttt tttggtttgc agtcagagta gaatagaagt atcaaaaaaa gcaccgactc
     1861 ggtgccactt tttcaagttg ataacggact agccttattt taacttgcta tgctgttttg
     1921 aatggttcca acaagattat tttataactt ttataacaaa taatcaagga gaaattcaaa
     1981 gaaatttatc agccataaaa caatacttaa tactatagaa tgataacaaa ataaactact
     2041 ttttaaaaga attttgtgtt ataatctatt tattattaag tattgggtaa tattttttga
     2101 agagatattt tgaaaaagaa aaattaaagc atattaaact aatttcggag gtcattaaaa
     2161 ctattattga acaaattgtt ggcagactga acctgatttc agtatcatgc ccagtcattt
     2221 cttcacctgt ggagcttttt aagtatggca cgcgtaactg ttcaggacgc tgtagagaaa
     2281 attggtaacc gttttgacct ggtactggtc gccgcgcgtc gcgctcgtca gatgcaggta
     2341 ggcggaaagg atccgctggt accggaagaa aacgataaaa ccactgtaat cgcgctgcgc
     2401 gaaatcgaag aaggtctgat caacaaccag atcctcgacg ttcgcgaacg ccaggaacag
     2461 caagagcagg aagccgctga attacaagcc gttaccgcta ttgctgaagg tcgtcgtgct
     2521 gcagataaga aatactcaat aggcttagct atcggcacaa atagcgtcgg atgggcggtg
     2581 atcactgatg aatataaggt tccgtctaaa aagttcaagg ttctgggaaa tacagaccgc
     2641 cacagtatca aaaaaaatct tataggggct cttttatttg acagtggaga gacagcggaa
     2701 gcgactcgtc tcaaacggac agctcgtaga aggtatacac gtcggaagaa tcgtatttgt
     2761 tatctacagg agattttttc aaatgagatg gcgaaagtag atgatagttt ctttcatcga
     2821 cttgaagagt cttttttggt ggaagaagac aagaagcatg aacgtcatcc tatttttgga
     2881 aatatagtag atgaagttgc ttatcatgag aaatatccaa ctatctatca tctgcgaaaa
     2941 aaattggtag attctactga taaagcggat ttgcgcttaa tctatttggc cttagcgcat
     3001 atgattaagt ttcgtggtca ttttttgatt gagggagatt taaatcctga taatagtgat
     3061 gtggacaaac tatttatcca gttggtacaa acctacaatc aattatttga agaaaaccct
     3121 attaacgcaa gtggagtaga tgctaaagcg attctttctg cacgattgag taaatcaaga
     3181 cgattagaaa atctcattgc tcagctcccc ggtgagaaga aaaatggctt atttgggaat
     3241 ctcattgctt tgtcattggg tttgacccct aattttaaat caaattttga tttggcagaa
     3301 gatgctaaat tacagctttc aaaagatact tacgatgatg atttagataa tttattggcg
     3361 caaattggag atcaatatgc tgatttgttt ttggcagcta agaatttatc agatgctatt
     3421 ttactttcag atatcctaag agtaaatact gaaataacta aggctcccct atcagcttca
     3481 atgattaaac gctacgatga acatcatcaa gacttgactc ttttaaaagc tttagttcga
     3541 caacaacttc cagaaaagta taaagaaatc ttttttgatc aatcaaaaaa cggatatgca
     3601 ggttatattg atgggggagc tagccaagaa gaattttata aatttatcaa accaatttta
     3661 gaaaaaatgg atggtactga ggaattattg gtgaaactaa atcgtgaaga tttgctgcgc
     3721 aagcaacgga cctttgacaa cggctctatt ccccatcaaa ttcacttggg tgagctgcat
     3781 gctattttga gaagacaaga agacttttat ccatttttaa aagacaatcg tgagaagatt
     3841 gaaaaaatct tgacttttcg aattccttat tatgttggtc cattggcgcg tggcaatagt
     3901 cgttttgcat ggatgactcg gaagtctgaa gaaacaatta ccccatggaa ttttgaagaa
     3961 gttgtcgata aaggtgcttc agctcaatca tttattgaac gcatgacaaa ctttgataaa
     4021 aatcttccaa atgaaaaagt actaccaaaa catagtttgc tttatgagta ttttacggtt
     4081 tataacgaat tgacaaaggt caaatatgtt actgaaggaa tgcgaaaacc agcatttctt
     4141 tcaggtgaac agaagaaagc cattgttgat ttactcttca aaacaaatcg aaaagtaacc
     4201 gttaagcaat taaaagaaga ttatttcaaa aaaatagaat gttttgatag tgttgaaatt
     4261 tcaggagttg aagatagatt taatgcttca ttaggtacct accatgattt gctaaaaatt
     4321 attaaagata aagatttttt ggataatgaa gaaaatgaag atatcttaga ggatattgtt
     4381 ttaacattga ccttatttga agatagggag atgattgagg aaagacttaa aacatatgct
     4441 cacctctttg atgataaggt gatgaaacag cttaaacgtc gccgttatac tggttgggga
     4501 cgtttgtctc gaaaattgat taatggtatt agggataagc aatctggcaa aacaatatta
     4561 gattttttga aatcagatgg ttttgccaat cgcaatttta tgcagctgat ccatgatgat
     4621 agtttgacat ttaaagaaga cattcaaaaa gcacaagtgt ctggacaagg cgatagttta
     4681 catgaacata ttgcaaattt agctggtagc cctgctatta aaaaaggtat tttacagact
     4741 gtaaaagttg ttgatgaatt ggtcaaagta atggggcggc ataagccaga aaatatcgtt
     4801 attgaaatgg cacgtgaaaa tcagacaact caaaagggcc agaaaaattc gcgagagcgt
     4861 atgaaacgaa tcgaagaagg tatcaaagaa ttaggaagtc agattcttaa agagcatcct
     4921 gttgaaaata ctcaattgca aaatgaaaag ctctatctct attatctcca aaatggaaga
     4981 gacatgtatg tggaccaaga attagatatt aatcgtttaa gtgattatga tgtcgatgcc
     5041 attgttccac aaagtttcct taaagacgat tcaatagaca ataaggtctt aacgcgttct
     5101 gataaaaatc gtggtaaatc ggataacgtt ccaagtgaag aagtagtcaa aaagatgaaa
     5161 aactattgga gacaacttct aaacgccaag ttaatcactc aacgtaagtt tgataattta
     5221 acgaaagctg aacgtggagg tttgagtgaa cttgataaag ctggttttat caaacgccaa
     5281 ttggttgaaa ctcgccaaat cactaagcat gtggcacaaa ttttggatag tcgcatgaat
     5341 actaaatacg atgaaaatga taaacttatt cgagaggtta aagtgattac cttaaaatct
     5401 aaattagttt ctgacttccg aaaagatttc caattctata aagtacgtga gattaacaat
     5461 taccatcatg cccatgatgc gtatctaaat gccgtcgttg gaactgcttt gattaagaaa
     5521 tatccaaaac ttgaatcgga gtttgtctat ggtgattata aagtttatga tgttcgtaaa
     5581 atgattgcta agtctgagca agaaataggc aaagcaaccg caaaatattt cttttactct
     5641 aatatcatga acttcttcaa aacagaaatt acacttgcaa atggagagat tcgcaaacgc
     5701 cctctaatcg aaactaatgg ggaaactgga gaaattgtct gggataaagg gcgagatttt
     5761 gccacagtgc gcaaagtatt gtccatgccc caagtcaata ttgtcaagaa aacagaagta
     5821 cagacaggcg gattctccaa ggagtcaatt ttaccaaaaa gaaattcgga caagcttatt
     5881 gctcgtaaaa aagactggga tccaaaaaaa tatggtggtt ttgatagtcc aacggtagct
     5941 tattcagtcc tagtggttgc taaggtggaa aaagggaaat cgaagaagtt aaaatccgtt
     6001 aaagagttac tagggatcac aattatggaa agaagttcct ttgaaaaaaa tccgattgac
     6061 tttttagaag ctaaaggata taaggaagtt aaaaaagact taatcattaa actacctaaa
     6121 tatagtcttt ttgagttaga aaacggtcgt aaacggatgc tggctagtgc cggagaatta
     6181 caaaaaggaa atgagctggc tctgccaagc aaatatgtga attttttata tttagctagt
     6241 cattatgaaa agttgaaggg tagtccagaa gataacgaac aaaaacaatt gtttgtggag
     6301 cagcataagc attatttaga tgagattatt gagcaaatca gtgaattttc taagcgtgtt
     6361 attttagcag atgccaattt agataaagtt cttagtgcat ataacaaaca tagagacaaa
     6421 ccaatacgtg aacaagcaga aaatattatt catttattta cgttgacgaa tcttggagct
     6481 cccgctgctt ttaaatattt tgatacaaca attgatcgta aacgatatac gtctacaaaa
     6541 gaagttttag atgccactct tatccatcaa tccatcactg gtctttatga aacacgcatt
     6601 gatttgagtc agctaggagg tgactgaagt atattttaga tgaagattat ttcttaataa
     6661 ctaaaaatat ggtataatac tcttaataaa tgcagtaata caggggcttt tcaagactga
     6721 agtctagctg agacaaatag tgcgattacg aaatttttta gacaaaaata gtctacgagg
     6781 ttttagagct atgctgtttt gaatggtccc aaaactgaga ccagtctcgg aagctcaaag
     6841 gtctcgtttt agagctatgc tgttttgaat ggtcccaaaa cttcagcaca ctgagacttg
     6901 ttgagttcca tgttttagag ctatgctgtt ttgaatggac tccattcaac attgccgatg
     6961 ataacttgag aaagagggtt aataccagca gtcggatacc ttcctattct ttctgttaaa
     7021 gcgttttcat gttataatag gcaaaagaag agtagtgtga tcgtccattc cgacagcatc
     7081 gccagtcact atggcgtgct gctagcgcta tatgcgttga tgcaatttct atgcgcaccc
     7141 gttctcggag cactgtccga ccgctttggc cgccgcccag tcctgctcgc ttcgctactt
     7201 ggagccacta tcgactacgc gatcatggcg accacacccg tcctgtggat cctctacgcc
     7261 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
     7321 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
     7381 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
     7441 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
     7501 caggagtcgc ataagggaga gcgtcgaccg atgcccttga gagccttcaa cccagtcagc
     7561 tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc
     7621 atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt
     7681 cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc
     7741 gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc
     7801 gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg
     7861 atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag
     7921 gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg
     7981 gctcttacca gcctaacttc gatcattgga ccgctgatcg tcacggcgat ttatgccgcc
     8041 tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc
     8101 ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc
     8161 ggcacctcgc taacggattc accactccaa gaattggagc caatcaattc ttgcggagaa
     8221 ctgtgaatgc gcaaaccaac ccttggcaga acatatccat cgcgtccgcc atctccagca
     8281 gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc
     8341 tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat
     8401 caccgatacg cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa
     8461 caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtccccta
     8521 cgtgctgctg aagttgcccg caacagagag tggaaccaac cggtgatacc acgatactat
     8581 gactgagagt caacgccatg agcggcctca tttcttattc tgagttacaa cagtccgcac
     8641 cgctgtccgg tagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga
     8701 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgcccaac agtcccccgg
     8761 ccacggggcc tgccaccata cccacgccga aacaagcgcc ctgcaccatt atgttccgga
     8821 tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta ttaacgaagc
     8881 gctaaccgtt tttatcaggc tctgggaggc agaataaatg atcatatcgt caattattac
     8941 ctccacgggg agagcctgag caaactggcc tcaggcattt gagaagcaca cggtcacact
     9001 gcttccggta gtcaataaac cggtaaacca gcaatagaca taagcggcta tttaacgacc
     9061 ctgccctgaa ccgacgaccg ggtcgaattt gctttcgaat ttctgccatt catccgctta
     9121 ttatcactta ttcaggcgta gcaccaggcg tttaagggca ccaataactg ccttaaaaaa
     9181 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     9241 catggaagcc atcacagacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     9301 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     9361 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     9421 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     9481 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     9541 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     9601 caccgtcttt cattgccata cg
//
