LOCUS       sequence-65851-e         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Conway DE, Breckenridge MT, Hinde E, Gratton E, Chen CS, Schwartz MA
  TITLE     Fluid Shear Stress on Endothelial Cells Modulates Mechanical Tension
            across VE-Cadherin and PECAM-1.
  JOURNAL   Curr Biol. 2013 May 14. pii: S0960-9822(13)00490-9. doi: 
            10.1016/j.cub.2013.04.049.
  PUBMED    23684974
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2013 May 14. pii: S0960-9822(13)00490-9. doi: 
            10.1016/j.cub.2013.04.049."
COMMENT     Sequence Label: pLPCX-PECAMTS
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(153..860)
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcacc atagcaccac ctggacctgc accacctgga
       61 cctgcaccac ctggacctgc accaccgggc cctgcaccac ctggccctgc accacctggc
      121 cctgcaccac ctggccctgc accacctggc cccttgtaca gctcgtccat gccgtcggtg
      181 gagttgcggg ccacggcgct ctcgtaaacg gtcaccttgt tgtagtcctt gtcgtggttc
      241 aggatctcga tgcggtggtc cacaaagtga tagtcgggca gcttcaccgc cttcttggcc
      301 ctgtagatgg tcttgaagtc aacgcggtgg tggccgccgc cctccagcag cagcttgtgc
      361 ttgacgtcgc ccttcagcac gccgtcgcgc acgtacatcc tctcggtgga ggcgtcccag
      421 ccggtggtct tcttctgcat cacggggccg ttggggggga agttctcgcc cttgaggtgt
      481 atctcgtaga tgaaggagtc ctcctccatg gagatgtcgg acttcacctt cacgatgccc
      541 ttgtcctcga aggtcatggt gcgctcccaa gagtagccct cggggaagga ctgcttgaag
      601 tagttgggga tgtcgtcggg gtacttggtg aaggccctgt tgccgtaggc gaacgcggtg
      661 gtcagaatgt cgtaggagaa gggcaggggg gctccctcct tcacctccag gttgatggtg
      721 ttggtgccgt cgtagggctt gccctcgccc tcgccctcga tcacgaaggc gtggccattc
      781 acgttgccct ccatcttcag cttgatcttc atgtcgggct tgattacgcc cattgtggtc
      841 tcctcgccct tgctcaccat ctcgacagct ttccggactt cactgtacac tgtctctgtg
      901 tccttctttc ctagatcttt gtgagactca gctgaggaca cttgaacttc cgtgtactgc
      961 acgtctgagt tca
//
