LOCUS       sequence-65855-e         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Conway DE, Breckenridge MT, Hinde E, Gratton E, Chen CS, Schwartz MA
  TITLE     Fluid Shear Stress on Endothelial Cells Modulates Mechanical Tension
            across VE-Cadherin and PECAM-1.
  JOURNAL   Curr Biol. 2013 May 14. pii: S0960-9822(13)00490-9. doi: 
            10.1016/j.cub.2013.04.049.
  PUBMED    23684974
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2013 May 14. pii: S0960-9822(13)00490-9. doi: 
            10.1016/j.cub.2013.04.049."
COMMENT     Sequence Label: pLPCX-VEcadTS
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(151..858)
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 ggtgaacagc tcctcgccct tgctcaccat agcaccacct ggacctgcac cacctggacc
       61 tgcaccacct ggacctgcac caccgggccc tgcaccacct ggccctgcac cacctggccc
      121 tgcaccacct ggccctgcac cacctggccc cttgtacagc tcgtccatgc cgtcggtgga
      181 gttgcgggcc acggcgctct cgtaaacggt caccttgttg tagtccttgt cgtggttcag
      241 gatctcgatg cggtggtcca caaagtgata gtcgggcagc ttcaccgcct tcttggccct
      301 gtagatggtc ttgaagtcaa cgcggtggtg gccgccgccc tccagcagca gcttgtgctt
      361 gacgtcgccc ttcagcacgc cgtcgcgcac gtacatcctc tcggtggagg cgtcccagcc
      421 ggtggtcttc ttctgcatca cggggccgtt gggggggaag ttctcgccct tgaggtgtat
      481 ctcgtagatg aaggagtcct cctccatgga gatgtcggac ttcaccttca cgatgccctt
      541 gtcctcgaag gtcatggtgc gctcccaaga gtagccctcg gggaaggact gcttgaagta
      601 gttggggatg tcgtcggggt acttggtgaa ggccctgttg ccgtaggcga acgcggtggt
      661 cagaatgtcg taggagaagg gcaggggggc tccctccttc acctccaggt tgatggtgtt
      721 ggtgccgtcg tagggcttgc cctcgccctc gccctcgatc acgaaggcgt ggccattcac
      781 gttgccctcc atcttcagct tgatcttcat gtcgggcttg attacgccca ttgtggtctc
      841 ctcgcccttg ctcaccatct cgacaccgcg cacagaatta agcactgaca catcatagct
      901 ggtggtgtcc atctcaccac cgccctcctc atcgtaagtg accaactgct cgtgaatctc
      961 cagcgcactc ttgctatga
//
