LOCUS       sequence-66114-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Hackel BJ, Kapila A, Wittrup KD
  TITLE     Picomolar affinity fibronectin domains engineered utilizing loop 
            length diversity, recursive mutagenesis, and loop shuffling.
  JOURNAL   J Mol Biol. 2008 Sep 19;381(5):1238-52. doi: 
            10.1016/j.jmb.2008.06.051. Epub 2008 Jun 24.
  PUBMED    18602401
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2008.06.051"; journalName: "J Mol Biol"; date: 
            "2008-09-19- 19"; volume: "381"; issue: "5"; pages: "1238-52"
COMMENT     Sequence Label: pCT-L7.5.1
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(18..36)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    77..580
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        617..721
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aacgcgcgag acgaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacggatc gcttgcctgt aacttacacg cgcctcgtat cttttaatga
      121 tggaataatt tgggaattta ctctgtgttt atttattttt atgttttgta tttggatttt
      181 agaaagtaaa taaagaaggt agaagagtta cggaatgaag aaaaaaaaat aaacaaaggt
      241 ttaaaaaatt tcaacaaaaa gcgtacttta catatatatt tattagacaa gaaaagcaga
      301 ttaaatagat atacattcga ttaacgataa gtaaaatgta aaatcacagg attttcgtgt
      361 gtggtcttct acacagacaa gatgaaacaa ttcggcatta atacctgaga gcaggaagag
      421 caagataaaa ggtagtattt gttggcgatc cccctagagt cttttacatc ttcggaaaac
      481 aaaaactatt ttttctttaa tttctttttt tactttctat ttttaattta tatatttata
      541 ttaaaaaatt taaattataa ttatttttat agcacgtgat gaaaaggacc caggtggcac
      601 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      661 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      721 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      781 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
      841 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atc
//
