LOCUS       sequence-66117-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE
  TITLE     One-step cloning and chromosomal integration of DNA.
  JOURNAL   ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. 
            Epub 2013 May 20.
  PUBMED    24050148
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400021j";
            journalName: "ACS Synth Biol"; date: "2013-09-20- 20"; volume: "2"; 
            issue: "9"; pages: "537-41"
COMMENT     Sequence Label: pOSIP-CH (CamR, HK022)
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(52..154)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
       61 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
      121 atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc agatatccac
      181 aacgtgcgtt acatatcaaa gggaaaactg tccataccca tggtaagtag gtcattatta
      241 gtcaaaataa aatcatttgt cgatttcaat tttgtcccat tcccgtccac ggctgtccct
      301 ataccgcgcc gccattgaat ctgatttatg cccgagaaga cgttgagcaa acttatcgcc
      361 aatctggttc cggtatagcc tcgctgacag gctacgcagt tcatggaatg ttggcgggtt
      421 tccatcaaat gagagtccag atgcatttct cgcctttgta aaatactttg atactgtttt
      481 cggggaaagc ggatcgtgat gctttgatgc gattatagtt tcactgctgc tggcctccct
      541 gcattgctgt agtgtatcag ccaatgagat attgagcgcg tcaatcgtta gcgttagtgg
      601 aatggcgagt ttagcccctg ttttactctg ttcaatgtga agatggttgt cgtttatgtc
      661 tgaccatttc attctgcaca aatcgcctac tctctgccct gtaacgacag ccaaatccat
      721 tgccagtctc agccagattg ggagcggttc ggctgcatgg tatatcgcga catattcatt
      781 agctgtcagt cttgaacgcc ttacttctga ttttgctgtg cgggttgctg ttaccggatt
      841 cgtagccaca tgcccctcgg ctattgcctc acgaaaaacg tcaacaaggg ttgacctgat
      901 caattttgcg ga
//
