LOCUS       sequence-66119-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE
  TITLE     One-step cloning and chromosomal integration of DNA.
  JOURNAL   ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. 
            Epub 2013 May 20.
  PUBMED    24050148
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400021j";
            journalName: "ACS Synth Biol"; date: "2013-09-20- 20"; volume: "2"; 
            issue: "9"; pages: "537-41"
COMMENT     Sequence Label: pOSIP-CH (CamR, HK022)
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(362..448)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(545..631)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(728..814)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(855..873)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctgacagcag acgtgcactg gccaggggga tcaccatccg tcgcccgggc gtgtcaataa
       61 tatcactctg tacatccaca aacagacgat aacggctctc tcttttatag gtgtaaacct
      121 taaactgcat agcatacctc tttttgacat acttcgggta tacatatcag tatatattct
      181 tataccgcaa aaatcagcgc gcaaatacgc atactgttat ctggctttta gtaagccctc
      241 tagaggatcc ccgggtaccg agctcgaatt ccgatcccca attcctggca gtttatggcg
      301 ggcgtcctgc ccgccaccct ccgggccgtt gcttcgcaac gttcaaatcc gctcccggcg
      361 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
      421 ctttcgactg agcctttcgt tttatttgat gcctggggaa ttaattcctg gcagtttatg
      481 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcgc aacgttcaaa tccgctcccg
      541 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
      601 agtctttcga ctgagccttt cgttttattt gatgcctggg gaattaattc ctggcagttt
      661 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cgcaacgttc aaatccgctc
      721 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
      781 cccagtcttt cgactgagcc tttcgtttta tttgatgcct ggggaattgg gaattaattc
      841 ttgaagacga aagggcctcg tgatacgcct atttttatag g
//
