LOCUS       sequence-66120-e         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE
  TITLE     One-step cloning and chromosomal integration of DNA.
  JOURNAL   ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. 
            Epub 2013 May 20.
  PUBMED    24050148
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400021j";
            journalName: "ACS Synth Biol"; date: "2013-09-20- 20"; volume: "2"; 
            issue: "9"; pages: "537-41"
COMMENT     Sequence Label: pOSIP-CH (CamR, HK022)
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    120..140
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      195..222
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(271..302)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     protein_bind    379..412
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
       61 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatt
      121 actagtagcg gccgctgcag gcatgcctcg agatgcatgg cgcctaacct aaactgacag
      181 gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc gaacaattga
      241 aaaaacctcg cgccttacct gttgagtaat agtcaaaagc ctccggtcgg aggcttttga
      301 ctttctgctt actgaatttc ggtggtgccg ttaattaacc ggtgggccct catgataata
      361 atggtttctt agacgtccga agttcctatt ctctagaaag tataggaact tccctaggtc
      421 agccaaacgt ctcttcaggc cactgactag cgataacttt ccccacaacg gaacaactct
      481 cattgcatgg gatcattggg tactgtgggt ttagtggttg taaaaacacc tgaccgctat
      541 ccctgatcag tttcttgaag gtaaactcat cacccccaag tctggctatg cagaaatcac
      601 ctggctcaac agcctgctca gggtcaacga gaattaacat tccgtcagga aagcttggct
      661 tggagcctgt tggtgcggtc atggaattac cttcaacctc aagccagaat gcagaatcac
      721 tggctttttt ggttgtgctt acccatctct ccgcatcacc tttggtaaag gttctaagct
      781 taggtgagaa catccctgcc tgaacatgag aaaaaacagg gtactcatac tcacttctaa
      841 gtgacggctg catactaacc gcttcataca tctcgtagat ttctctggcg attgaagggc
      901 taaattcttc aacgctaact ttgagaattt ttgtaagcaa tgcggcgtta taagcattta
      961 atg
//
