LOCUS       sequence-66123-e         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE
  TITLE     One-step cloning and chromosomal integration of DNA.
  JOURNAL   ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. 
            Epub 2013 May 20.
  PUBMED    24050148
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400021j";
            journalName: "ACS Synth Biol"; date: "2013-09-20- 20"; volume: "2"; 
            issue: "9"; pages: "537-41"
COMMENT     Sequence Label: pOSIP-CT (CamR, P21)
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(52..154)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
       61 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
      121 atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc agatatccac
      181 aacgtgcgtt acatatcaaa gggaaaactg tccataccca tggataacgg gcgtataaca
      241 cgcccgttgt tttatgatga tgtaaaatct tccccaaaac tttccccaaa acccttcccc
      301 aaaactggct attttctatg ctgttttgat atctacgata atccagtctt taccacgatc
      361 atcattgtat cggtcggtca tttttctgga tttatggcct aacaactttt gcgtatccag
      421 accctgttcc cgatataatc gttctgacag agatcgctgt tcatgaaatg tgggcgcagt
      481 tccttgctcc cattttatgc cacatttttc cttggccttt ttaaaagccg ttgtcagagt
      541 atttgcagaa acctggtctc ctctgtttgc ttgagaggta gtgtgacggt aatggaccag
      601 atatttacta acaacagcat ccctgcactg agatataact tcacgaaggg taatattcag
      661 agcatcgcat ttcaggttaa gcggaatagc aagttttgaa ccggtttttt cctgagtaat
      721 gtgcaacatg tcgtcccata tatcagagaa tttcaaattg cagatatcgc ctaaacgttg
      781 tccagtaaca agagcaagta gcatgccgca ttttaaatag ggctgccgtc tgcttacgct
      841 ttcaaatatt gcctgccatt cgagcagtga caatctttgg cggtttactc gatttcgcgg
      901 ttgttttgtt gcctgcgctg ggttaaatcc tggcggaaca tgtcctgcgt gttgtgcttc
      961 tttgaagacg tcgatcaaca ccatt
//
