LOCUS       sequence-66124-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE
  TITLE     One-step cloning and chromosomal integration of DNA.
  JOURNAL   ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. 
            Epub 2013 May 20.
  PUBMED    24050148
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400021j";
            journalName: "ACS Synth Biol"; date: "2013-09-20- 20"; volume: "2"; 
            issue: "9"; pages: "537-41"
COMMENT     Sequence Label: pOSIP-KC (KanR, PhiC31)
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    129..149
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      204..231
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(280..311)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     protein_bind    388..421
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
       61 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
      121 gctcacatta ctagtagcgg ccgctgcagg catgcctcga gatgcatggc gcctaaccta
      181 aactgacagg catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtttcg
      241 aacaattgaa aaaacctcgc gccttacctg ttgagtaata gtcaaaagcc tccggtcgga
      301 ggcttttgac tttctgctta ctgaatttcg gtggtgccgt taattaaccg gtgggccctc
      361 atgataataa tggtttctta gacgtccgaa gttcctattc tctagaaagt ataggaactt
      421 ccctaggtca gccaaacgtc tcttcaggcc actgactagc gataactttc cccacaacgg
      481 aacaactctc attgcatggg atcattgggt actgtgggtt tagtggttgt aaaaacacct
      541 gaccgctatc cctgatcagt ttcttgaagg taaactcatc acccccaagt ctggctatgc
      601 agaaatcacc tggctcaaca gcctgctcag ggtcaacgag aattaacatt ccgtcaggaa
      661 agcttggctt ggagcctgtt ggtgcggtca tggaattacc ttcaacctca agccagaatg
      721 cagaatcact ggcttttttg gttgtgctta cccatctctc cgcatcacct ttggtaaagg
      781 ttctaagctt aggtgagaac atccctgcct gaacatgaga aaaaacaggg tactcatact
      841 cacttctaag tgacggctgc atacta
//
