LOCUS       sequence-66125-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE
  TITLE     One-step cloning and chromosomal integration of DNA.
  JOURNAL   ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. 
            Epub 2013 May 20.
  PUBMED    24050148
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400021j";
            journalName: "ACS Synth Biol"; date: "2013-09-20- 20"; volume: "2"; 
            issue: "9"; pages: "537-41"
COMMENT     Sequence Label: pOSIP-KC (KanR, PhiC31)
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 tccatgttgg agtttaagcg cggacgggag caagacgttt cccgttgaat atggctcata
       61 acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt
      121 ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtgtaagta ggtcattatt
      181 agtcaaaata aaactacgcc gctacgtctt ccgtgccgtc ctgggcgtcg tcttcgtcgt
      241 cgtcggtcgg cggcttcgcc cacgtgatcg aagcgcgctt ctcgatgggc gttccctgcc
      301 ccctgcccgt agtcgacttc gtgacaacga tcttgtctac gaagagcccg acgaacacgc
      361 gcttgtcgtc tactgacgcg cgcccccacc acgacttagg gccggtcggg tcagcgtcgg
      421 cgtcttcggg gaaccattgg tcaaggggaa gcttcggggc ttcggcggct tcaagttcgg
      481 caagccgctc ttccgcccct tgctgccgga gcgtcagcgc tgcctgttgc ttccggaagt
      541 gcttcctgcc aacgggtccg tcgtacgcgc ctgccgcgcg gtcttcgtac agctcttcaa
      601 gggcgttcag ggcgtcggcg cgctccgcaa caaggttcgc ccgttcgccg ctcttctcag
      661 gcgcctcagt gagcttgccg aagcgtcggg cggcttccca cagaagcgcc aacgtctctt
      721 cgtcgccttc ggcgtgcctg atcttgttga agatgcgttc cgcaacgaac ttgtcgagtg
      781 ccgccatgct gacgttgcac gtgccttcgt gctgcccagg tgcggacggg tcgaccacct
      841 tccggcgacg gcagcggtaa gagtccttga tcgattcttc cccgcgcttc gaagtcatga
      901 cggcgccaca ctcgcagtac agcttgtcca tggcggacag aatggcttgc ccccgggaaa
      961 gccccttg
//
