LOCUS       sequence-66127-e         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE
  TITLE     One-step cloning and chromosomal integration of DNA.
  JOURNAL   ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. 
            Epub 2013 May 20.
  PUBMED    24050148
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400021j";
            journalName: "ACS Synth Biol"; date: "2013-09-20- 20"; volume: "2"; 
            issue: "9"; pages: "537-41"
COMMENT     Sequence Label: pOSIP-KH (KanR, HK022)
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 cgtccatgtt ggagtttaag cgcggacggg agcaagacgt ttcccgttga atatggctca
       61 taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat
      121 ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtgcgtt acatatcaaa
      181 gggaaaactg tccataccca tggtaagtag gtcattatta gtcaaaataa aatcatttgt
      241 cgatttcaat tttgtcccat tcccgtccac ggctgtccct ataccgcgcc gccattgaat
      301 ctgatttatg cccgagaaga cgttgagcaa acttatcgcc aatctggttc cggtatagcc
      361 tcgctgacag gctacgcagt tcatggaatg ttggcgggtt tccatcaaat gagagtccag
      421 atgcatttct cgcctttgta aaatactttg atactgtttt cggggaaagc ggatcgtgat
      481 gctttgatgc gattatagtt tcactgctgc tggcctccct gcattgctgt agtgtatcag
      541 ccaatgagat attgagcgcg tcaatcgtta gcgttagtgg aatggcgagt ttagcccctg
      601 ttttactctg ttcaatgtga agatggttgt cgtttatgtc tgaccatttc attctgcaca
      661 aatcgcctac tctctgccct gtaacgacag ccaaatccat tgccagtctc agccagattg
      721 ggagcggttc ggctgcatgg tatatcgcga catattcatt agctgtcagt cttgaacgcc
      781 ttacttctga ttttgctgtg cgggttgctg ttaccggatt cgtagccaca tgcccctcgg
      841 ctattgcctc acgaaaaacg tcaacaaggg ttgacctgat caattttgcg gaagccgatt
      901 tgccttctgc tacgtaggtg tttagcattg ctgccacctc tttcgttgat atgtcagcga
      961 gcggtttgt
//
