LOCUS       sequence-66135-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bicistronic reporter plasmid expressing Renilla luciferase and 
            firefly luciferase in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Poulin F, Gingras AC, Olsen H, Chevalier S, Sonenberg N
  TITLE     4E-BP3, a new member of the eukaryotic initiation factor 4E-binding 
            protein family.
  JOURNAL   J Biol Chem. 1998 May 29;273(22):14002-7.
  PUBMED    9593750
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1998-05-29"; volume: "273(22)"; pages: "14002-7"
COMMENT     Sequence Label: pcDNA3 RLUC POLIRES FLUC
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttgggct agccaccatg acttcgaaag tttatgatcc
      121 agaacaaagg aaacggatga taactggtcc gcagtggtgg gccagatgta aacaaatgaa
      181 tgttcttgat tcatttatta attattatga ttcagaaaaa catgcagaaa atgctgttat
      241 ttttttacat ggtaacgcgg cctcttctta tttatggcga catgttgtgc cacatattga
      301 gccagtagcg cggtgtatta taccagacct tattggtatg ggcaaatcag gcaaatctgg
      361 taatggttct tataggttac ttgatcatta caaatatctt actgcatggt ttgaacttct
      421 taatttacca aagaagatca tttttgtcgg ccatgattgg ggtgcttgtt tggcatttca
      481 ttatagctat gagcatcaag ataagatcaa agcaatagtt cacgctgaaa gtgtagtaga
      541 tgtgattgaa tcatgggatg aatggcctga tattgaagaa gatattgcgt tgatcaaatc
      601 tgaagaagga gaaaaaatgg ttttggagaa taacttcttc gtggaaacca tgttgccatc
      661 aaaaatcatg agaaagttag aaccagaaga atttgcagca tatcttgaac cattcaaaga
      721 gaaaggtgaa gttcgtcgtc caacattatc atggcctcgt gaaatcccgt tagtaaaagg
      781 tggtaaacct gacgttgtac aaattgttag gaattataat gcttatctac gtgcaagtga
      841 tgatttacca aaaatgttta ttgaatcgga cccaggattc ttttccaatg ct
//
