LOCUS       sequence-66141-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Oliveira AF, Yasuda R
  TITLE     An improved Ras sensor for highly sensitive and quantitative 
            FRET-FLIM imaging.
  JOURNAL   PLoS One. 2013;8(1):e52874. doi: 10.1371/journal.pone.0052874. Epub 
            2013 Jan 14.
  PUBMED    23349692
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(1):e52874. doi"; pages: " 
            10.1371/journal.pone.0052874. Epub 2013 Jan 14"
COMMENT     Sequence Label: FRas2-F Acceptor
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(338..356)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     606..625
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     800..823
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 atgagcttgc atgactgcct tatgaaagca ctcaaggtga ggggcctgca accagagtgc
       61 tgtgcagtgt tcagacttct ccacgaacac aaaggtgcaa aagcacgctt agattggaat
      121 actgatgctg cgtctttgat tggagaagaa cttcaagtag atttcctggg aattctgcag
      181 tcgacggtac cgcgggcccg ggatccaatg gcctcctccg aggacgtcat caaggagttc
      241 atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      301 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
      361 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tccagtacgg ctccaaggcc
      421 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      481 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      541 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
      601 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga gcggatgtac
      661 cccgaggacg gcgccctgaa gggcgagatc aagatgaggc tgaagctgaa ggacggcggc
      721 cactacgacg ccgaggtcaa gaccacctac atggccaaga agcccgtgca gctgcccggc
      781 gcctacaaga ccgacatcaa gctggacatc acctcccaca acgaggacta c
//
