LOCUS       sequence-66147-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Oliveira AF, Yasuda R
  TITLE     An improved Ras sensor for highly sensitive and quantitative 
            FRET-FLIM imaging.
  JOURNAL   PLoS One. 2013;8(1):e52874. doi: 10.1371/journal.pone.0052874. Epub 
            2013 Jan 14.
  PUBMED    23349692
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(1):e52874. doi"; pages: " 
            10.1371/journal.pone.0052874. Epub 2013 Jan 14"
COMMENT     Sequence Label: FRas2-M Acceptor
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             214..888
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             214..888
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(344..362)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     612..631
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     806..829
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 aatggaatga gcttgcatga ctgccttatg aaagcactca aggtgagggg cctgcaacca
       61 gagtgctgtg cagtgttcag acttctccac gaacacaaag gtgcaaaagc acgcttagat
      121 tggaatactg atgctgcgtc tttgattgga gaagaacttc aagtagattt cctgggaatt
      181 ctgcagtcga cggtaccgcg ggcccgggat ccaatggcct cctccgagga cgtcatcaag
      241 gagttcatgc gcttcaaggt gcgcatggag ggctccgtga acggccacga gttcgagatc
      301 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
      361 aagggcggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcca gtacggctcc
      421 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
      481 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
      541 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
      601 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgg
      661 atgtaccccg aggacggcgc cctgaagggc gagatcaaga tgaggctgaa gctgaaggac
      721 ggcggccact acgacgccga ggtcaagacc acctacatgg ccaagaagcc cgtgcagctg
      781 cccggcgcct acaagaccga catcaagctg gacatcacct cccacaacga ggactacacc
      841 atcgtggaac agtacgagcg cgccgagggc cgccactcca ccggcgcct
//
