LOCUS       sequence-66148-e         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Dunlop MJ, Dossani ZY, Szmidt HL, Chu HC, Lee TS, Keasling JD, Hadi 
            MZ, Mukhopadhyay A
  TITLE     Engineering microbial biofuel tolerance and export using efflux 
            pumps.
  JOURNAL   Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
  PUBMED    21556065
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2011-05-10"; volume: "7:487. doi"; pages: " 
            10.1038/msb.2011.21"
COMMENT     Sequence Label: pBbA5k-EPL66
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..20)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tggtgatggt gatgatgatg cgctggctgc gcgtgctcgc cgtcaccctt gtcagccgct
       61 tcgcgacgct gttcgtcggt tggttccttg atgcggtacc aggccacgta caacgcaggc
      121 aggaatagca gggtcagcaa cgtggcgatg atgatgccac cgatcatggc gtaggccatc
      181 gggccccaga acacttcccg cgcaataggg atcatgccca ggctcgccgc ggccgcggtc
      241 agcaaaatcg gacgacggcg atgctcggtg gcttcgacca cagcgtccca aggcagatac
      301 ccactgcgct cataggcatc gatctgtgtc accagaatca ccgagttgcg gatgatgatg
      361 ccgatcagcg ccagcacccc gagaatcgcc acgaacccca gtggcgtccc cgtcgggatc
      421 agcgccagca ccacaccgat tagccccaga ggcgcgacac tggccaccag aaacatcttc
      481 tgaatgctgt gcaactgaat catcaggaac gtcgccatga ggaacagcat cagcggcgca
      541 acgctggcaa tcggcccctg cgccttgctg ctttcttcga ccgtgccgcc cgttgcaacc
      601 ttgtagccga ccggcagacc agcggcgaac ttgtcgattt caggttgcag ctgtttgacc
      661 agatcggttg gctgaatcgc gtcccgtacc gcgcccttga cggtgatggt cggcatgcga
      721 tcgcggcgcc agaccaacgg ctgctccagc tcgtaaccaa cggtggcgaa cgccagcagc
      781 ggaatggatg ttccagtttg ggt
//
