LOCUS       sequence-66153-e         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE
  TITLE     One-step cloning and chromosomal integration of DNA.
  JOURNAL   ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. 
            Epub 2013 May 20.
  PUBMED    24050148
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400021j";
            journalName: "ACS Synth Biol"; date: "2013-09-20- 20"; volume: "2"; 
            issue: "9"; pages: "537-41"
COMMENT     Sequence Label: pOSIP-KO (KanR, 186)
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    125..145
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      200..227
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(276..307)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     protein_bind    384..417
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
       61 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      121 acattactag tagcggccgc tgcaggcatg cctcgagatg catggcgcct aacctaaact
      181 gacaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttcgaaca
      241 attgaaaaaa cctcgcgcct tacctgttga gtaatagtca aaagcctccg gtcggaggct
      301 tttgactttc tgcttactga atttcggtgg tgccgttaat taaccggtgg gccctcatga
      361 taataatggt ttcttagacg tccgaagttc ctattctcta gaaagtatag gaacttccct
      421 aggtcagcca aacgtctctt caggccactg actagcgata actttcccca caacggaaca
      481 actctcattg catgggatca ttgggtactg tgggtttagt ggttgtaaaa acacctgacc
      541 gctatccctg atcagtttct tgaaggtaaa ctcatcaccc ccaagtctgg ctatgcagaa
      601 atcacctggc tcaacagcct gctcagggtc aacgagaatt aacattccgt caggaaagct
      661 tggcttggag cctgttggtg cggtcatgga attaccttca acctcaagcc agaatgcaga
      721 atcactggct tttttggttg tgcttaccca tctctccgca tcacctttgg taaaggttct
      781 aagcttaggt gagaacatcc ctgcctgaac atgagaaaaa acagggtact catactcact
      841 tctaagtgac ggctgcatac taaccgcttc atacatctcg tagatttctc tggcgattga
      901 agggctaaat tcttcaacgc taactttgag aatttttgta agcaatgcgg cgttataagc
      961 atttaatgca ttgatgccat taaataaagc accaac
//
