LOCUS       sequence-66158-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  TITLE     Yeast Secretion Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRS316-4M5.3
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(21..39)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    80..582
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        619..723
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
       61 aataatggtt tcttagacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa
      121 tgatggaata atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat
      181 tttagaaagt aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa
      241 ggtttaaaaa atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc
      301 agattaaata gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg
      361 tgtgtggtct tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa
      421 gagcaagata aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa
      481 aacaaaaact attttttctt taatttcttt tttactttct atttttaatt tatatattta
      541 tattaaaaaa tttaaattat aattattttt atagcacgtg atgaaaagga cccaggtggc
      601 acttttcggg gaaatgtgcg cgaaacccct atttgtttat ttttctaaat acattcaaat
      661 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      721 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      781 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
      841 gcac
//
