LOCUS       sequence-66159-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE
  TITLE     One-step cloning and chromosomal integration of DNA.
  JOURNAL   ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. 
            Epub 2013 May 20.
  PUBMED    24050148
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400021j";
            journalName: "ACS Synth Biol"; date: "2013-09-20- 20"; volume: "2"; 
            issue: "9"; pages: "537-41"
COMMENT     Sequence Label: pOSIP-KO (KanR, 186)
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 tccatgttgg agtttaagcg cggacgggag caagacgttt cccgttgaat atggctcata
       61 acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt
      121 ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtgcgttac atatcaaagg
      181 gaaaactgtc catacccatg ggctagccga actgacatac aacctccata acttatttgc
      241 tgttagtaat gtgaactaaa ggatttagtt taactgcatc ctctaaatgg tcgggtgcaa
      301 agtgcgcata tcgcatggtc atttttatat ctgtatggcc gagtacgcgc tgcaacacca
      361 aaatattacc gccattcatc ataaagtgac tagcgaaggt gtgacgtaaa acgtgggtaa
      421 gttgtcctgc cggtagttcg atacctgttc tttccaaagc tgaccggaac gcgccataac
      481 aatcactgaa caaccggccc tttttatcat caggcagaga ctcatagagc tctttgctga
      541 ttgggacggt gcgatttttt ctacctttcg tgttggtgta tgtgattttg tatttcgcga
      601 gttggctttt tctcagactc tcggcctcag accaccgtgc gccagttgcg agacagattc
      661 ttaccacggt ttctaaatca gggtggtcat gtcggttaca ctctccgagc agttgcgaaa
      721 tttggtcgtg agttagccaa gtcatttcca tttcttctgt gcggaatggg cgcatatttt
      781 ttagtgggtt ttcacccttc cattctccga ggcggtttag ctcattgaac accgcccgga
      841 agtaggccag ctcaagatta agcgtgcgag gcgatacctc tttcactctg tttga
//
