LOCUS       sequence-66160-e         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE
  TITLE     One-step cloning and chromosomal integration of DNA.
  JOURNAL   ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. 
            Epub 2013 May 20.
  PUBMED    24050148
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400021j";
            journalName: "ACS Synth Biol"; date: "2013-09-20- 20"; volume: "2"; 
            issue: "9"; pages: "537-41"
COMMENT     Sequence Label: pOSIP-KP (KanR, Phi80)
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 cgtccatgtt ggagtttaag cgcggacggg agcaagacgt ttcccgttga atatggctca
       61 taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat
      121 ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtgcgtt acatatcaaa
      181 gggaaaactg tccataccca tggcaatcaa agagtgggag gataaaaaga agataaatta
      241 actgaagggc tctccgatga tctcttcaag atggctctgc caaacgcgga gccagtgttt
      301 ctgatcatcg atatagtcat gaaggttgta atgcgccata acccccacca tctgatgccc
      361 gagcagcttt tcaattacgt gcggcgggca acctaactca gagagatttg tggctatcgt
      421 ccgcctcata tcatgaagcg accactctgc catacctgtt ccattccaaa tagaacgggc
      481 gtaattggat gccacaggtg aatgaacggg cgaatctttg atcccgccat caattttacg
      541 ttgtgaagtc accaggtgat tggtgtttat tttcttgagg tgatttctga ccaggttaac
      601 ggcggcgtct gagagtcccc ttctaatatg tacccgagtt ttataactgc ccgcaggcac
      661 gacccactca ttatcatcca atcgaaacca tgatctctca ctaagtcgaa tctcagccgt
      721 acggcatccg gtaagcataa taaatttcac caggaaaacg gactctatcg acatatggct
      781 tttcaaccac tgatagattt tgcgcagatc gtcatcgtcc atcctgcgag ttctcttttt
      841 aggcttttgc ccgacatcag atggcagtaa tccctcgagt gggtttgagg cgatcacact
      901 tctgttaacg cagaacctaa acgcccgttt g
//
