LOCUS       sequence-66163-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE
  TITLE     One-step cloning and chromosomal integration of DNA.
  JOURNAL   ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. 
            Epub 2013 May 20.
  PUBMED    24050148
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400021j";
            journalName: "ACS Synth Biol"; date: "2013-09-20- 20"; volume: "2"; 
            issue: "9"; pages: "537-41"
COMMENT     Sequence Label: pOSIP-KP (KanR, Phi80)
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(372..458)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(555..641)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(738..824)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tctgacagca gacgtgcact ggccaggggg atcaccatct gtcgcccggg cgtgtcaata
       61 atatcactct gtacatccac aaacagacga taacggctct ctcttttata ggtgtaaacc
      121 ttaaactgca tagcatacct ctttttgaca tacttcgggt atacatatca gtatatattc
      181 ttataccgca aaaatcagcg cgcaagtacg catactgtta tctggctttt agtaagccct
      241 ctagaagcgg ccgcggatcc ccgggtaccg agctcgaatt ccgatcccca attcctggca
      301 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcgcaac gttcaaatcc
      361 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      421 aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctggggaa ttaattcctg
      481 gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcgc aacgttcaaa
      541 tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa
      601 cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcctggg gaattaattc
      661 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cgcaacgttc
      721 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
      781 aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct ggggaa
//
