LOCUS       sequence-66165-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE
  TITLE     One-step cloning and chromosomal integration of DNA.
  JOURNAL   ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. 
            Epub 2013 May 20.
  PUBMED    24050148
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400021j";
            journalName: "ACS Synth Biol"; date: "2013-09-20- 20"; volume: "2"; 
            issue: "9"; pages: "537-41"
COMMENT     Sequence Label: pOSIP-KP (KanR, Phi80)
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    129..149
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      198..225
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(274..305)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     protein_bind    382..415
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
       61 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
      121 gctcacatta ctagtagcgg ccgctgcagc tcgagatgca tggcgcctaa cctaaactga
      181 caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt ttcgaacaat
      241 tgaaaaaacc tcgcgcctta cctgttgagt aatagtcaaa agcctccggt cggaggcttt
      301 tgactttctg cttactgaat ttcggtggtg ccgttaatta accggtgggc cctcatgata
      361 ataatggttt cttagacgtc cgaagttcct attctctaga aagtatagga acttccctag
      421 gtcagccaaa cgtctcttca ggccactgac tagcgataac tttccccaca acggaacaac
      481 tctcattgca tgggatcatt gggtactgtg ggtttagtgg ttgtaaaaac acctgaccgc
      541 tatccctgat cagtttcttg aaggtaaact catcaccccc aagtctggct atgcagaaat
      601 cacctggctc aacagcctgc tcagggtcaa cgagaattaa cattccgtca ggaaagcttg
      661 gcttggagcc tgttggtgcg gtcatggaat taccttcaac ctcaagccag aatgcagaat
      721 cactggcttt tttggttgtg cttacccatc tctccgcatc acctttggta aaggttctaa
      781 gcttaggtga gaacatccct gcctgaacat gagaaaaaac agggtactca tactcacttc
      841 taagtgacgg ctgcatacta accgcttcat acatctcgta gatttctctg gcgattgaag
      901 ggctaaattc ttcaacgcta a
//
