LOCUS       sequence-66171-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE
  TITLE     One-step cloning and chromosomal integration of DNA.
  JOURNAL   ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. 
            Epub 2013 May 20.
  PUBMED    24050148
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400021j";
            journalName: "ACS Synth Biol"; date: "2013-09-20- 20"; volume: "2"; 
            issue: "9"; pages: "537-41"
COMMENT     Sequence Label: pOSIP-TH (TetR, HK022)
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        165..219
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    166..184
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ttaacgcata aagtgcaagc aatacgccaa agtggttagc gatatcttcc gaagcaataa
       61 attcacgtaa taacgttggc aagactggca tgataaggcc aatccccatg gcatcgagca
      121 acgtaattac caatgcgatc tttgtcgaac tattcatttc acttttctct atcactgata
      181 gggagtggga aaataactct atcaatgata gggtgtcaaa tcgatgcaca acgtgcgtta
      241 catatcaaag ggaaaactgt ccatacccat ggtaagtagg tcattattag tcaaaataaa
      301 atcatttgtc gatttcaatt ttgtcccatt cccgtccacg gctgtcccta taccgcgccg
      361 ccattgaatc tgatttatgc ccgagaagac gttgagcaaa cttatcgcca atctggttcc
      421 ggtatagcct cgctgacagg ctacgcagtt catggaatgt tggcgggttt ccatcaaatg
      481 agagtccaga tgcatttctc gcctttgtaa aatactttga tactgttttc ggggaaagcg
      541 gatcgtgatg ctttgatgcg attatagttt cactgctgct ggcctccctg cattgctgta
      601 gtgtatcagc caatgagata ttgagcgcgt caatcgttag cgttagtgga atggcgagtt
      661 tagcccctgt tttactctgt tcaatgtgaa gatggttgtc gtttatgtct gaccatttca
      721 ttctgcacaa atcgcctact ctctgccctg taacgacagc caaatccatt gccagtctca
      781 gccagattgg gagcggttcg gctgcatggt atatcgcgac atattcatta gctgtcagtc
      841 ttgaacgcct tacttctgat tttgctgtgc gggttgctgt taccggattc gta
//
