LOCUS       sequence-66172-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE
  TITLE     One-step cloning and chromosomal integration of DNA.
  JOURNAL   ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. 
            Epub 2013 May 20.
  PUBMED    24050148
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400021j";
            journalName: "ACS Synth Biol"; date: "2013-09-20- 20"; volume: "2"; 
            issue: "9"; pages: "537-41"
COMMENT     Sequence Label: pOSIP-TH (TetR, HK022)
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(363..449)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(546..632)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(729..815)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(856..874)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tctgacagca gacgtgcact ggccaggggg atcaccatcc gtcgcccggg cgtgtcaata
       61 atatcactct gtacatccac aaacagacga taacggctct ctcttttata ggtgtaaacc
      121 ttaaactgca tagcatacct ctttttgaca tacttcgggt atacatatca gtatatattc
      181 ttataccgca aaaatcagcg cgcaaatacg catactgtta tctggctttt agtaagccct
      241 ctagaggatc cccgggtacc gagctcgaat tccgatcccc aattcctggc agtttatggc
      301 gggcgtcctg cccgccaccc tccgggccgt tgcttcgcaa cgttcaaatc cgctcccggc
      361 ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag
      421 tctttcgact gagcctttcg ttttatttga tgcctgggga attaattcct ggcagtttat
      481 ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttcg caacgttcaa atccgctccc
      541 ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc
      601 cagtctttcg actgagcctt tcgttttatt tgatgcctgg ggaattaatt cctggcagtt
      661 tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcgcaacgtt caaatccgct
      721 cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag
      781 gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tggggaattg ggaattaatt
      841 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgtgcagca
      901 tacactc
//
