LOCUS       sequence-66174-e        1105 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE
  TITLE     One-step cloning and chromosomal integration of DNA.
  JOURNAL   ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. 
            Epub 2013 May 20.
  PUBMED    24050148
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400021j";
            journalName: "ACS Synth Biol"; date: "2013-09-20- 20"; volume: "2"; 
            issue: "9"; pages: "537-41"
COMMENT     Sequence Label: pOSIP-TT (TetR, P21)
FEATURES             Location/Qualifiers
     source          1..1105
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        168..222
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    169..187
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gcattaacgc ataaagtgca agcaatacgc caaagtggtt agcgatatct tccgaagcaa
       61 taaattcacg taataacgtt ggcaagactg gcatgataag gccaatcccc atggcatcga
      121 gcaacgtaat taccaatgcg atctttgtcg aactattcat ttcacttttc tctatcactg
      181 atagggagtg ggaaaataac tctatcaatg atagggtgtc aaatcgatgc acaacgtgcg
      241 ttacatatca aagggaaaac tgtccatacc catggataac gggcgtataa cacgcccgtt
      301 gttttatgat gatgtaaaat cttccccaaa actttcccca aaacccttcc ccaaaactgg
      361 ctattttcta tgctgttttg atatctacga taatccagtc tttaccacga tcatcattgt
      421 atcggtcggt catttttctg gatttatggc ctaacaactt ttgcgtatcc agaccctgtt
      481 cccgatataa tcgttctgac agagatcgct gttcatgaaa tgtgggcgca gttccttgct
      541 cccattttat gccacatttt tccttggcct ttttaaaagc cgttgtcaga gtatttgcag
      601 aaacctggtc tcctctgttt gcttgagagg tagtgtgacg gtaatggacc agatatttac
      661 taacaacagc atccctgcac tgagatataa cttcacgaag ggtaatattc agagcatcgc
      721 atttcaggtt aagcggaata gcaagttttg aaccggtttt ttcctgagta atgtgcaaca
      781 tgtcgtccca tatatcagag aatttcaaat tgcagatatc gcctaaacgt tgtccagtaa
      841 caagagcaag tagcatgccg cattttaaat agggctgccg tctgcttacg ctttcaaata
      901 ttgcctgcca ttcgagcagt gacaatcttt ggcggtttac tcgatttcgc ggttgttttg
      961 ttgcctgcgc tgggttaaat cctggcggaa catgtcctgc gtgttgtgct tctttgaaga
     1021 cgtcgatcaa caccattctc acgacttgcg ccatcctgtt atgaccttca gcctttacag
     1081 catcaattat ttcggcaata tcaag
//
