LOCUS       sequence-66179-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Ponchon L, Catala M, Seijo B, El Khouri M, Dardel F, Nonin-Lecomte 
            S, Tisne C
  TITLE     Co-expression of RNA-protein complexes in Escherichia coli and 
            applications to RNA biology.
  JOURNAL   Nucleic Acids Res. 2013 Aug;41(15):e150. doi: 10.1093/nar/gkt576. 
            Epub 2013 Jun 26.
  PUBMED    23804766
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2013-08"; volume: "41(15):e150. doi"; pages: " 
            10.1093/nar/gkt576. Epub 2013 Jun 26"
COMMENT     Sequence Label: p44K
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(198..302)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(334..379)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(571..588)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(642..664)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    653..669
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(677..683,684..701,702..707))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    722..743
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
       61 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
      121 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
      181 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
      241 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
      301 cgcacatttc cccgaaaagt gccacctgac gtaaaaaaac ccctcaagac ccgtttagag
      361 gccccaaggg gttatgctaa agcttaaaaa aaatccttag ctttcgctaa ggatctgcag
      421 tggtggaggc ggcgggaatc gaacccgcgt ccgaggacac gtccgaagcc acagagacac
      481 ggcctgcgga cgtcgcagcc gaatctacgt cgaccgcagg gccgtctccc tgtggtgtcc
      541 ccgcgaatcc tttccgcccc cgaattctta gtggtgatgg tgatgatggg taccggcggc
      601 cgcctcgagg gatccactag tgccatggcc catatgtgtt tcctgtgtga aattgttatc
      661 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
      721 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
      781 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
      841 ttgggcgctc ttccgcttcc tcgctcac
//
