LOCUS       sequence-66183-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Kebache S, Zuo D, Chevet E, Larose L
  TITLE     Modulation of protein translation by Nck-1.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5406-11.
  PUBMED    11959995
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2002-04-16"; volume: "99(8)"; pages: 
            "5406-11"
COMMENT     Sequence Label: pcDNA3-HA-Nck1
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             123..149
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag cgtttaaact taagcttact agtggtacca
      121 tgtatgatgt tcctgattat gctagcctcg gatccatggc agaagaagtg gtggtagtag
      181 ccaaatttga ttatgtggcc caacaagaac aagagttgga catcaagaag aatgagagat
      241 tatggcttct ggatgattct aagtcctggt ggcgagttcg aaattccatg aataaaacag
      301 gttttgtgcc ttctaactat gtggaaagga aaaacagtgc tcggaaagca tctattgtga
      361 aaaacctaaa ggatacctta ggcattggaa aagtgaaaag aaaacctagt gtgccagatt
      421 ctgcatctcc tgctgatgat agttttgttg acccagggga acgtctctat gacctcaaca
      481 tgcccgctta tgtgaaattt aactacatgg ctgagagaga ggatgaatta tcattgataa
      541 aggggacaaa ggtgatcgtc atggagaaat gcagtgatgg gtggtggcgt ggtagctaca
      601 atggacaagt tggatggttc ccttcaaact atgtaactga agaaggtgac agtcctttgg
      661 gtgaccatgt gggttctctg tcagagaaat tagcagcagt cgtcaataac ctaaatactg
      721 ggcaagtgtt gcatgtggta caggctcttt acccattcag ctcatctaat gatgaagaac
      781 ttaatttcga gaaaggagat gtaatggatg ttattgaaaa acctgaaaat gacccagagt
      841 ggtggaaatg caggaagatc aatggtatgg ttggtc
//
