LOCUS       sequence-66185-e        1047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Kebache S, Zuo D, Chevet E, Larose L
  TITLE     Modulation of protein translation by Nck-1.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5406-11.
  PUBMED    11959995
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2002-04-16"; volume: "99(8)"; pages: 
            "5406-11"
COMMENT     Sequence Label: pSV HA eIF2beta WT
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     95..114
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        95..113
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             142..168
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ctctggctaa
       61 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
      121 aagctggcta gtggtaccat gtatgatgtt cctgattatg ctagcctcgg atccatgtcc
      181 ggggacgaga tgatttttga tcctactatg agcaaaaaga aaaagaagaa gaagaagcct
      241 tttatgttag atgaagaagg tgatgcccag acagaagaaa cccagccctc agagacaaaa
      301 gaagtggagc cagaaccaac tgaagaaaaa gacgtggacg ctgatgagga agacagtagg
      361 aagaaagatg cttctgatga cttagatgat ttgaacttct ttaatcaaaa gaaaaagaag
      421 aaaaagacaa aaaagatatt tgacattgat gaagctgaag aagctataaa ggatgttaag
      481 attgagagtg atgctcaaga gccagccgag ccagaggatg accttgacat tatgcttggc
      541 aacaaaaaaa agaaaaagaa gaatgttaaa ttcccagaag aagatgaaat actagaaaaa
      601 gacgaagctt tagaagatga agacagtaaa aaagatgatg gtatttcatt cagtagccaa
      661 actgcttggg caggctcaga aagagactac acatatgagg agttgctgaa ccgagtgttc
      721 aacatcatga gagaaaagaa tccagatatg gttgctggag agaagaggaa atttgttatg
      781 aaacctccac aggtcgtccg agtaggaacc aagaaaactt cttttgtcaa ttttacagat
      841 atctgtaaac tattacatcg tcaacccaaa catctccttg catttttatt ggcagaattg
      901 ggtaccagtg gttctataga tggtaataac caacttgtaa tcaaaggaag attccaacag
      961 aaacaaatag aaaatgtctt gagaagatat atcaaggaat atgtcacttg tcacacatgc
     1021 cggtcaccgg acacaatcct acagaag
//
