LOCUS       sequence-66193-e         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, 
            Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel 
            AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P,
            Mesirov JP, Beroukhim R, Hahn WC
  TITLE     Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer
            drivers.
  JOURNAL   Cancer Discov. 2013 Jun 13. [Epub ahead of print]
  PUBMED    23764425
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2013 Jun 13. [Epub ahead of print]"
COMMENT     Sequence Label: pLEX_970_puro_DEST_YFP
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(82..123)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    125..149
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(149..862,863..865,866..868))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(194..215)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     543..562
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     802..823
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(868..892)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(915..966)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(944..964)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt gtcgacttaa cgcgccaccg
       61 gttagcgcta gctcattact acgtagaatc gagaccgagg agagggttag ggataggctt
      121 accaaccact ttgtacaaga aagttgggtt acttgtacag ctcgtccatg ccgagagtga
      181 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
      241 tgctcagggc ggactggtag ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
      301 cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt
      361 ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt
      421 tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt
      481 cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc
      541 gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg
      601 gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg gcgaagcact
      661 gcaggccgta gccgaaggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg
      721 tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca
      781 cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg
      841 tgaacagctc ctcgcccttg ctcaccatgc caactttttt gtacaaactt gttgatcccg
      901 acagttagcc agagagctct gcttatatag acctcccacc gtacacgcct accgcccatt
      961 tgcgtcaatg gggcgga
//
