LOCUS       sequence-66196-e        1027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1027)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ELAC1
FEATURES             Location/Qualifiers
     source          1..1027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(98..121)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(122..139)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(140..160)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgacggagc tcgaattcgg atcctcactt atcgtcgtca tccttgtaat
      121 catggtggtg atgatggtgc gattggaagt agaggttctc tgctttcttg attggaatgc
      181 ttatcaccat aaaatcttct gctagagtca cttcttggag atctaacact gattcagctt
      241 gcttttttag ttctgcaatg ccatctgttt ctccttctct ggccaaggca actggtttgt
      301 acctctgact gaagtgagtc agaaccagcc tctttgcacg gcacaacttt gcaaatgttg
      361 ctgccatctg tggtgtgctg tggccatgct cctttgcttt gtccatctgg gcatcatcca
      421 gggttgcttc gtggatcaac aggtctgctt caaagcacag ttttactcct ccatcaccca
      481 caaccccaga gcagtcaccc aatatgcaga tttttcttcc aacaataggc ttttttaaga
      541 catcttgggg agaaattgta accccatttt ccagaacaac agaaattcca tttttcagct
      601 tcccataggc aggacctggt ggaacaccaa ggtctttaag tttctgtgca ttgagtttac
      661 ctgggcgttt cttttccacg actgaaaacc caaatgaggg aattctgtga aagaggcgaa
      721 atgcttttac aacaaattgt tcatcatcaa acagaaggta tgagttttct tctgagtcta
      781 acaggatagt tcttccttgt tcctctttgg gaggactgtc tgctctattc acatgcgcaa
      841 attcttttag ttcttctgca ggacattgat ctgctgtagg aaccagttca tgaaccacat
      901 aatggaagac cagctccgtg tgagagagtt ccatggttcg ccagataaag tcccgaagcc
      961 ctacagggcc atagatttca ataggctgtt tggacaccat ggagccactc tgcaggctga
     1021 ttgtgca
//
