LOCUS       sequence-66197-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, 
            Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel 
            AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P,
            Mesirov JP, Beroukhim R, Hahn WC
  TITLE     Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer
            drivers.
  JOURNAL   Cancer Discov. 2013 Jun 13. [Epub ahead of print]
  PUBMED    23764425
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2013 Jun 13. [Epub ahead of print]"
COMMENT     Sequence Label: pLEX_970_puro_DEST_V5_hcRED
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(82..123)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    125..149
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(151..834)
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     protein_bind    complement(837..861)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt gtcgacttaa cgcgccaccg
       61 gttagcgcta gctcattact acgtagaatc gagaccgagg agagggttag ggataggctt
      121 accaaccact ttgtacaaga aagttggcaa gttggccttc tcgggcaggt cgctgtaccg
      181 ggccacgctg gcctcgtaca gctcgaagta ctcgtccttc ttcttccgca gcatctggag
      241 ccggatgtcg gtgaagtgga agccgggcat ggtcagggcg cgcacggcct tcttgctccg
      301 gtagctggtg tagtggtggc agatcaggtg ccggtcgccc accttcaggg ccatcacgtt
      361 ccggccgcac agcacgccgt tctcggggta caccacctcg gtgctgggct cccagccgcc
      421 gctcttgttc ttcatcacgg ggccgtcggc ggggaagttg gtgccgtgca ccttcacctt
      481 gtagatcagg cagttgccct ccaggctggt gtcctggtgg gcggtcagga tgccgccgtc
      541 ctcgtaggtg gtggttctct cccaggtgaa gccctcgggg aagctctgct tgaagaagtc
      601 ggggatctcg gcggtgtggt gcacgaaggt cctgctgccg tactcgcagc agggggccag
      661 gatgtcgaag gcgaagggca ggggggcgcc ctcggtcacg tggattctca tgctctgggt
      721 gccggcgaag gggttgccgt cgccctcgcc ctcgcacttg aagtagtggc cgttcacggt
      781 gccctccatg tacatcttga tgcgcatact ctccttcagc aggccgctca ccatggtgcc
      841 aacttttttg tacaaacttg ttgatcccga cagttagcca gagagctctg cttatata
//
