LOCUS       sequence-66200-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, 
            Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel 
            AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P,
            Mesirov JP, Beroukhim R, Hahn WC
  TITLE     Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer
            drivers.
  JOURNAL   Cancer Discov. 2013 Jun 13. [Epub ahead of print]
  PUBMED    23764425
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2013 Jun 13. [Epub ahead of print]"
COMMENT     Sequence Label: pBabe_puro_DEST_Flag_MDS1
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             38..79
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             104..127
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    134..158
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(666..690)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(702..722)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 cggatcccag tgtggtggta cgtaggaatt cgccaccatg gggtcttcaa aatctaaacc
       61 aaaggacccc agccagcgcc ggcgcaggat ccgaggttac cttgactaca aagacgatga
      121 cgacaagcaa ttgacaagtt tgtacaaaaa agttggcatg agatccaaag gcagggcaag
      181 gaaactggcc acaaataatg agtgtgtata tggcaactac cctgaaatac ctttggaaga
      241 aatgccagat gcagatggag tagccagcac tccctccctc aatattcaag agccatgctc
      301 tcctgccaca tccagtgaag cattcactcc aaaggagggt tctccttaca aagcccccat
      361 ctacatccct gatgatatcc ccattcctgc tgagtttgaa cttcgagagt caaatatgcc
      421 tggggcagga ctaggaatat ggaccaaaag gaagatcgaa gtaggtgaaa agtttgggcc
      481 ttatgtggga gagcagaggt caaacctgaa agaccccagt tatggatggg aggtacatct
      541 tccaaggtct cggagggtaa gcgttcactc ttggttgtat ttggggaaga gaagctcaga
      601 cgtaggaata gccttctctc aggctgatgt ctacatgcct ggactgcagt gtgccttcct
      661 ctcgtaccca actttcttgt acaaagtggt ctcgaccctg tggaatgtgt gtcagttagg
      721 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
      781 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
      841 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
      901 tccgcccagt t
//
