LOCUS       sequence-66204-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  TITLE     src plasmids
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCMV5 mouse src
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(11..524)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(206..227)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     418..437
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    570..794
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     783..800
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        820..838
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     820..837
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ctcggtaccc accccggttg ataatcagaa aagccccaaa aacaggaaga ttgtataagc
       61 aaatatttaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
      121 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
      181 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
      241 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
      301 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
      361 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
      421 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
      481 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtggggat cccccctcga
      541 gccccagctg gttctttccg cctcagaagc catagagccc accgcatccc cagcatgcct
      601 gctattgtct tcccaatcct cccccttgct gtcctgcccc accccacccc ccagaataga
      661 atgacaccta ctcagacaat gcgatgcaat ttcctcattt tattaggaaa ggacagtggg
      721 agtggcacct tccagggtca aggaaggcac gggggagggg caaacaacag atggctggca
      781 actagaaggc acagtcgagg ctgatcagcg agctctagca tttaggtgac actatagaat
      841 agggccctct agatgcatgc tcgagcggcc gcca
//
