LOCUS       sequence-66206-e        6305 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter gene for factors with GAL4 DNA binding domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Sun P, Enslen H, Myung PS, Maurer RA
  TITLE     Differential activation of CREB by Ca2+/calmodulin-dependent protein
            kinases type II and type IV involves phosphorylation of a site that 
            negatively regulates activity.
  JOURNAL   Genes Dev. 1994 Nov 1;8(21):2527-39.
  PUBMED    7958915
REFERENCE   2  (bases 1 to 6305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "1994-11-1"; volume: "8(21)"; pages: "2527-39"
COMMENT     Sequence Label: 5xGAL4-TATA-luciferase
FEATURES             Location/Qualifiers
     source          1..6305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    37..131
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     regulatory      213..222
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             219..1871
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(285..303)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          2003..2068
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2198..2218
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2643..2777
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3548..3566)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3634..3738
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3739..3807,3808..4599)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3957..3976)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4770..5358
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5259..5278
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5512..5529
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5544..5684
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5610..5629)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     polyA_signal    5934..6068
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    6171..6305
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 aattcgagct cggtaccagc ttgcatgcct gcaggtcgga gtactgtcct ccgagcggag
       61 tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac
      121 tgtcctccga gcggagactc tagagggtat ataatggatc ctggctagcc agtcgacctg
      181 caggcatgca agcttggcat tccggtactg ttgccaccat ggaagacgcc aaaaacataa
      241 agaaaggccc ggcgccattc tatcctctag aggatggaac cgctggagag caactgcata
      301 aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg
      361 aggtgaacat cacgtacgcg gaatacttcg aaatgtccgt tcggttggca gaagctatga
      421 aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat
      481 tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca
      541 tttataatga acgtgaattg ctcaacagta tgaacatttc gcagcctacc gtagtgtttg
      601 tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaaattacca ataatccaga
      661 aaattattat catggattct aaaactgatt accagggatt tcagtcgatg tacacgttcg
      721 tcacatctca tctacctccc ggttttaatg aatacgattt tgtaccagag tcctttgatc
      781 gtgacaaaac aattgcactg ataatgaatt cctctggatc tactgggtta cctaagggtg
      841 tggcccttcc gcatagaact gcctgcgtca gattctcgca tgccagagat cctatttttg
      901 gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat cacggttttg
      961 gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat
     1021 ttgaagaaga gctgttttta cgatcccttc aggattacaa aattcaaagt gcgttgctag
     1081 taccaaccct attttcattc ttcgccaaaa gcactctgat tgacaaatac gatttatcta
     1141 atttacacga aattgcttct gggggcgcac ctctttcgaa agaagtcggg gaagcggttg
     1201 caaaacgctt ccatcttcca gggatacgac aaggatatgg gctcactgag actacatcag
     1261 ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat
     1321 tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcagagag
     1381 gcgaattatg tgtcagagga cctatgatta tgtccggtta tgtaaacaat ccggaagcga
     1441 ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct tactgggacg
     1501 aagacgaaca cttcttcata gttgaccgct tgaagtcttt aattaaatac aaaggatatc
     1561 aggtggcccc cgctgaattg gaatcgatat tgttacaaca ccccaacatc ttcgacgcgg
     1621 gcgtggcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg
     1681 agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa
     1741 ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg
     1801 gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaagt
     1861 ccaaattgta aaatgtaact gtattcagcg atgacgaaat tcttagctat tgtaatgact
     1921 ctagaggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
     1981 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     2041 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     2101 gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga
     2161 tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga
     2221 agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa
     2281 tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa
     2341 gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa
     2401 catactgttt tttcttactc cacacaggca tagagtgtct gctattaata actatgctca
     2461 aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta
     2521 tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     2581 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     2641 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     2701 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     2761 cttatcatgt ctggatcgat ccccaggaag ctcctctgtg tcctcataaa ccctaacctc
     2821 ctctacttga gaggacattc caatcatagg ctgcccatcc accctctgtg tcctcctgtt
     2881 aattaggtca cttaacaaaa aggaaattgg gtaggggttt ttcacagacc gctttctaag
     2941 ggtaatttta aaatatctgg gaagtccctt ccactgctgt gttccagaag tgttggtaaa
     3001 cagcccacaa atgtcaacag cagaaacata caagctgtca gctttgcaca agggcccaac
     3061 accctgctca tcaagaagca ctgtggttgc tgtgttagta atgtgcaaaa caggaggcac
     3121 attttcccca cctgtgtagg ttccaaaata tctagtgttt tcatttttac ttggatcagg
     3181 aacccagcac tccactggat aagcattatc cttatccaaa acagccttgt ggtcagtgtt
     3241 catctgctga ctgtcaactg tagcattttt tggggttaca gtttgagcag gatatttggt
     3301 cctgtagttt gctaacacac cctgcagctc caaaggttcc ccaccaacag caaaaaaatg
     3361 aaaatttgac ccttgaatgg gttttccagc accattttca tgagtttttt gtgtccctga
     3421 atgcaagttt aacatagcag ttaccccaat aacctcagtt ttaacagtaa cagcttccca
     3481 catcaaaata tttccacagg ttaagtcctc atttaaatta ggcaaaggaa ttcttgaaga
     3541 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     3601 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     3661 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     3721 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     3781 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     3841 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     3901 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     3961 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     4021 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     4081 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     4141 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     4201 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     4261 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     4321 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     4381 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     4441 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     4501 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     4561 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     4621 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     4681 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     4741 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     4801 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     4861 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     4921 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     4981 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     5041 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     5101 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     5161 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     5221 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     5281 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     5341 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     5401 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     5461 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     5521 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     5581 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     5641 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
     5701 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     5761 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     5821 ggcagcggat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     5881 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     5941 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6001 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6061 tctggatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     6121 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     6181 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     6241 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     6301 ggatc
//
