LOCUS       sequence-66214-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Bar-Peled L, Chantranupong L, Cherniack AD, Chen WW, Ottina KA, 
            Grabiner BC, Spear ED, Carter SL, Meyerson M, Sabatini DM
  TITLE     A Tumor suppressor complex with GAP activity for the Rag GTPases 
            that signal amino acid sufficiency to mTORC1.
  JOURNAL   Science. 2013 May 31;340(6136):1100-6. doi: 10.1126/science.1232044.
  PUBMED    23723238
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2013-05-31- 31"; volume: "340"; 
            issue: "6136"; pages: "1100-6"
COMMENT     Sequence Label: C terminal Flag Mios pRK5
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(57..80)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttataagctg caataaacaa gttgggccat ggcggccaag ctggggatcc ttatcacttg
       61 tcatcgtcat ccttgtagtc acctccacct cctgcggccg ctggctggac agtctctgca
      121 ggtaccagat tccccgttgt atccaactgc atacatttac acgtgcatgc agacacaggg
      181 cactctgcat ggtccctgaa ccaactaagc atatgtccag catgtccacc gtgcctgcaa
      241 ttatgacacc atgtaaacca gttgttaaat tgggctaatt ttttgtcctt gctcaagtcc
      301 actttttcat ctgatttggt tcctccagga cagctagaaa ctggtgttcc catattaatg
      361 agacaaagcg cacatcgagg aagtggtttt cgacagccag gacaacttgt gactttagat
      421 ttcgttggtg agccactcac accatactga ctaaaacctc tgccctgatg aggcacagct
      481 gaacagctgt aggagattga cttgccacag aaattgcaac tcacaaaaac ttgtgctaaa
      541 ggcttggaac tgggatccaa cttactcctg tgaatatcaa attcagctcg tttatgccaa
      601 aacctccagg catctaataa atttctataa ttctcaatcc agtactgaac cctttcatct
      661 ttaagaacat ctaaaggtga accctgtaac atacagtaac ttgctgtttg aacatctcca
      721 gttctatcaa cataactctc cattaagtcc actccatctt tagtaaggcc tgtaagcaaa
      781 attccttcca aatttccagc ctctttcatt tcattggtca acttttcgat gtatctattt
      841 aactgagtat cactaaggaa tttacaa
//
