LOCUS       sequence-66225-e        5815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Gottardi CJ, Wong E, Gumbiner BM
  TITLE     E-cadherin suppresses cellular transformation by inhibiting 
            beta-catenin signaling in an adhesion-independent manner.
  JOURNAL   J Cell Biol. 2001 May 28;153(5):1049-60.
  PUBMED    11381089
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2001-05-28"; volume: "153(5)"; pages: "1049-60"
COMMENT     Sequence Label: IL2R/hE-cadherin-cytotail
FEATURES             Location/Qualifiers
     source          1..5815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        407..610
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     561..581
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     655..674
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        655..673
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          2048..2113
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2243..2263
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2688..2822
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2725..2744)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2779..2798
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3589..3607)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3675..3779
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3780..3848,3849..4640)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3998..4017)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4811..5399
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5300..5319
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5553..5570
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5651..5670)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac
      661 gactcactat agggagaccc aagctagctt ggcggctggt cccaagggtc aggaagatgg
      721 attcatacct gctgatgtgg ggactgctca cgttcatcat ggtgcctggc tgccaggcag
      781 agctctgtga cgatgacccg ccagagatcc cacacgccac attcaaagcc atggcctaca
      841 aggaaggaac catgttgaac tgtgaatgca agagaggttt ccgcagaata aaaagcgggt
      901 cactctatat gctctgtaca ggaaactcta gccactcgtc ctgggacaac caatgtcaat
      961 gcacaagctc tgccactcgg aacacaacga aacaagtgac acctcaacct gaagaacaga
     1021 aagaaaggaa aaccacagaa atgcaaagtc caatgcagcc agtggaccaa gcgagccttc
     1081 caggtcactg cagggaacct ccaccatggg aaaatgaagc cacagagaga atttatcatt
     1141 tcgtggtggg gcagatggtt tattatcagt gcgtccaggg atacagggct ctacacagag
     1201 gtcctgctga gagcgtctgc aaaatgaccc acgggaagac aaggtggacc cagccccagc
     1261 tcatatgcac aggtgaaatg gagaccagtc agtttccagg tgaagagaag cctcaggcaa
     1321 gccccgaagg ccgtcctgag agtgagactt cctgcctcgt cacaacaaca gattttcaaa
     1381 tacagacaga aatggctgca accatggaga cgtccatatt tacaacagag taccaggtag
     1441 cagtggccgg ctgtgttttc ctgctgatca gcgtcctcct cctgagtggg ctcacctgga
     1501 agcttcggag gagagcggtg gtcaaagagc ccttactgcc cccagaggat gacacccggg
     1561 acaacgttta ttactatgat gaagaaggag gcggagaaga ggaccaggac tttgacttga
     1621 gccagctgca caggggcctg gacgctcggc ctgaagtgac tcgtaacgac gttgcaccaa
     1681 ccctcatgag tgtcccccgg tatcttcccc gccctgccaa tcccgatgaa attggaaatt
     1741 ttattgatga aaatctgaaa gcggctgata ctgaccccac agccccgcct tatgattctc
     1801 tgctcgtgtt tgactatgaa ggaagcggtt ccgaagctgc tagtctgagc tccctgaact
     1861 cctcagagtc agacaaagac caggactatg actacttgaa cgaatggggc aatcgcttca
     1921 agaagctggc tgacatgtac ggaggcggcg aggaccacta ggggtctaga ggatctttgt
     1981 gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag
     2041 ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt
     2101 ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt
     2161 taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc
     2221 tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt
     2281 tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg
     2341 ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa
     2401 atattctgta acctttataa gtaggcataa cagttataat cataacatac tgttttttct
     2461 tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt
     2521 tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag
     2581 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     2641 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     2701 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     2761 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     2821 tccccaggaa gctcctctgt gtcctcataa accctaacct cctctacttg agaggacatt
     2881 ccaatcatag gctgcccatc caccctctgt gtcctcctgt taattaggtc acttaacaaa
     2941 aaggaaattg ggtaggggtt tttcacagac cgctttctaa gggtaatttt aaaatatctg
     3001 ggaagtccct tccactgctg tgttccagaa gtgttggtaa acagcccaca aatgtcaaca
     3061 gcagaaacat acaagctgtc agctttgcac aagggcccaa caccctgctc atcaagaagc
     3121 actgtggttg ctgtgttagt aatgtgcaaa acaggaggca cattttcccc acctgtgtag
     3181 gttccaaaat atctagtgtt ttcattttta cttggatcag gaacccagca ctccactgga
     3241 taagcattat ccttatccaa aacagccttg tggtcagtgt tcatctgctg actgtcaact
     3301 gtagcatttt ttggggttac agtttgagca ggatatttgg tcctgtagtt tgctaacaca
     3361 ccctgcagct ccaaaggttc cccaccaaca gcaaaaaaat gaaaatttga cccttgaatg
     3421 ggttttccag caccattttc atgagttttt tgtgtccctg aatgcaagtt taacatagca
     3481 gttaccccaa taacctcagt tttaacagta acagcttccc acatcaaaat atttccacag
     3541 gttaagtcct catttaaatt aggcaaagga attcttgaag acgaaagggc ctcgtgatac
     3601 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     3661 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     3721 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     3781 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     3841 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     3901 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     3961 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     4021 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     4081 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     4141 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     4201 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     4261 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     4321 ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     4381 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     4441 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     4501 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     4561 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     4621 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     4681 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     4741 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     4801 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4861 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4921 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     4981 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5041 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5101 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5161 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc
     5221 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     5281 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     5341 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     5401 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     5461 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     5521 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     5581 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     5641 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     5701 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     5761 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcg
//
