LOCUS       sequence-66230-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Dunlop MJ, Dossani ZY, Szmidt HL, Chu HC, Lee TS, Keasling JD, Hadi 
            MZ, Mukhopadhyay A
  TITLE     Engineering microbial biofuel tolerance and export using efflux 
            pumps.
  JOURNAL   Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
  PUBMED    21556065
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2011-05-10"; volume: "7:487. doi"; pages: " 
            10.1038/msb.2011.21"
COMMENT     Sequence Label: pBbA5k-EPL101
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..20)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tggtgatggt gatgatggtg actcgcttct ttcggctctt gctcctgagg ttgctcggac
       61 tctttaggca agcctttcgc cttttcacgc attttttgca tgattacgta aaacactggt
      121 accagcaggg tacctacgat agtggccgct aacataccgc caaacaccga gtaaccaagg
      181 gcacggcgac tccccgcccc cgcgcctgta gcgataacta aaggcaacac ccccaatagg
      241 aaagaaaaag ccgtcatcag caccgcacga aaacgcagcc ttgccgccgt ctgcgcagcg
      301 tcaaggatac tcttaccctc ttcgtgcaat tgtttagcaa actcaacaat caagatggca
      361 ttcttacagg cgaggccgat aagcagcacc agaccaatct gggcatagag gtttaaatcc
      421 gacccaacga gcaagatgtt gagaaatgcc cccagtaccg caataggtac tgcgagaata
      481 acggcaaatg gaatcgtcca actctcgtac tgcgccacta agaacaggta agtaaacact
      541 aatgccaacg agaaaataag cggcgctaga ttacccgctt tgatctcttg ataggtttgc
      601 cctgtccact cgaaggtata tcctgccgga agtgattcat tggcagcacg ctccatcgca
      661 gtaattgcat cacccgaact aaagccggga gcggggaagc cattgatagt tgtggaacta
      721 aacatgttgt agttattcat cacgtcaggc cccaaaattg gcgtcactgt caccaaagta
      781 cttaatggca ccatctcacc ggtgttagag cggacgtaga agcgcgagat atctc
//
