LOCUS       sequence-66231-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Ni TT, Lu J, Zhu M, Maddison LA, Boyd KL, Huskey L, Ju B, Hesselson 
            D, Zhong TP, Page-McCaw PS, Stainier DY, Chen W
  TITLE     Conditional control of gene function by an invertible gene trap in 
            zebrafish.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15389-94. Epub 2012 
            Aug 20.
  PUBMED    22908272
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-09-18"; volume: "109(38)"; pages: 
            "15389-94. Epub 2012 Aug 20"
COMMENT     Sequence Label: pFT1.0
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(230..277)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    complement(302..335)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    368..401
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    448..481
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     misc_feature    join(488..675,676..687)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(721..737)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(721..737)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(774..794)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(774..792)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(813..829)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(813..829)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(826..848)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    837..853
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggcctcgccc tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgtgcac
       61 cttgaagcgc atgaactcct tgatgatggc catgttatcc tcctcgccct tgctcaccat
      121 ggtggttctt ctcttcttcc gaccgcgaag agtttgtcga tcgacctgaa aaaaaaaagg
      181 gaagagagag acacgtcaga aacacacaca cactccggat tagtctcgag aagttcctat
      241 actttctaga gaataggaac ttcggaatag gaacttcaca cacacacaca cacacacaca
      301 cgaagttcct atactatttg aagaatagga acttcggaat aggaacttca ttaccctgtt
      361 atccctaata acttcgtata atgtatgcta tacgaagtta tccaagcatc accatcgacc
      421 ctctagtcca gatctcacca tcgacccata acttcgtata atgtgtacta tacgaagtta
      481 tgggcccaag tgatctccaa aaaataagta ctttttgact gtaaataaaa ttgtaaggag
      541 taaaaagtac ttttttttct aaaaaaatgt aattaagtaa aagtaaaagt attgattttt
      601 aattgtactc aagtaaagta aaaatcccca aaaataatac ttaagtacag taatcaagta
      661 aaattactca agtactttac acctctggtt cttgaccccc taccttcagc aagcccagca
      721 gatccactag ttctagagcg gccgccaccg cggtggagct ccagcttttg ttccctttag
      781 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
      841 tatccgctca caattccaca caacatacga gcc
//
