LOCUS       sequence-66235-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Boniface EJ, Lu J, Victoroff T, Zhu M, Chen W
  TITLE     FlEx-based transgenic reporter lines for visualization of Cre and 
            Flp activity in live zebrafish.
  JOURNAL   Genesis. 2009 Jul;47(7):484-91. doi: 10.1002/dvg.20526.
  PUBMED    19415631
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dvg"; 
            journalName: "Genesis"; date: "2009-07"; volume: "47"; issue: "7"; 
            pages: "484-91"
COMMENT     Sequence Label: pT2-eab2-[GTR]
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(216..263)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    complement(288..321)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    354..387
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    434..467
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(510..527)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    516..740
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgtgcac cttgaagcgc
       61 atgaactcct tgatgatggc catgttatcc tcctcgccct tgctcaccat ggtggttctt
      121 ctcttcttcc gaccgcgaag agtttgtcga cctgaaaaaa aaaagggaag agagagacac
      181 gtcagaaaca cacacacact ccggattagt ctcgagaagt tcctatactt tctagagaat
      241 aggaacttcg gaataggaac ttcacacaca cacacacaca cacacacgaa gttcctatac
      301 tatttgaaga ataggaactt cggaatagga acttcattac cctgttatcc ctaataactt
      361 cgtataatgt atgctatacg aagttatcca agcatcacca tcgaccctct agtccagatc
      421 tcaccatcga cccataactt cgtataatgt gtactatacg aagttatggg cccaagtgat
      481 ctccaaaaaa taagtaaacc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
      541 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
      601 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
      661 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
      721 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc aggatctaat actcaagtac
      781 aattttaatg
//
