LOCUS       sequence-66236-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Boniface EJ, Lu J, Victoroff T, Zhu M, Chen W
  TITLE     FlEx-based transgenic reporter lines for visualization of Cre and 
            Flp activity in live zebrafish.
  JOURNAL   Genesis. 2009 Jul;47(7):484-91. doi: 10.1002/dvg.20526.
  PUBMED    19415631
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dvg"; 
            journalName: "Genesis"; date: "2009-07"; volume: "47"; issue: "7"; 
            pages: "484-91"
COMMENT     Sequence Label: pT2-eab2-[GTR]
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(140..173)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    complement(219..252)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    279..312
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    359..392
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cgcccttgct caccatggtg gcgaccggtg gatccgtcga ggaattcttt gccaaaatga
       61 tgagacagca caacaaccag cacgttgccc aggagctgta ggaaagagaa gaaggcatga
      121 acatggttag cagaggtccg aagttcctat actatttgaa gaataggaac ttcggaatag
      181 gaacttcctg gccttggggg agggggaggc cagaatgaga agttcctata ctttctagag
      241 aataggaact tctaactata acggtcctaa ggtagcgaat aacttcgtat aatgtgtact
      301 atacgaagtt atgggtcgat ggtgatgctt ggcaattcgg gtcgatggtg aagcttggat
      361 aacttcgtat aatgtatgct atacgaagtt atgctagcta aagtaggtca caccctgctg
      421 cagcactgcc tgcacaaaga acttcccatg ccggctatcc atcaagtgtg caacatgctc
      481 accagcaccc tcaaaactgt agttaaaaat cacatttatg gctgttgaag agagattagc
      541 cagaacacaa ctttatatta agacttaaca cccatttcac accggcccat tcaatgttca
      601 atgtcgtgcc tacgactggt cacgttaaat taccgacatt ctcacctgcc acatccacta
      661 acgctacaaa aaaagttaag atacagccaa tatttattaa atgtaacaga tattatcaaa
      721 agaaccacca tttataaatc aaacctagta gataactaca aagagaccgt tatgaactgt
      781 ctaaagactt catcaaatca tatttatcca tttacacatt ttacttaaca ttaatagata
      841 gaaactataa ttaaacgctt aaa
//
