LOCUS       sequence-66238-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Boniface EJ, Lu J, Victoroff T, Zhu M, Chen W
  TITLE     FlEx-based transgenic reporter lines for visualization of Cre and 
            Flp activity in live zebrafish.
  JOURNAL   Genesis. 2009 Jul;47(7):484-91. doi: 10.1002/dvg.20526.
  PUBMED    19415631
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dvg"; 
            journalName: "Genesis"; date: "2009-07"; volume: "47"; issue: "7"; 
            pages: "484-91"
COMMENT     Sequence Label: pT2-eab2-[GTR]
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     12..32
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        14..32
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     69..85
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     69..85
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(119..130,131..318)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctc caccgcggtg
       61 gcggccgctc tagaactagt ggatctgctg ggcttgctga aggtaggggg tcaagaacca
      121 gaggtgtaaa gtacttgagt aattttactt gattactgta cttaagtatt atttttgggg
      181 atttttactt tacttgagta caattaaaaa tcaatacttt tacttttact taattacatt
      241 tttttagaaa aaaaagtact ttttactcct tacaatttta tttacagtca aaaagtactt
      301 attttttgga gatcacttgg gcccggctcg agcaggggga tcatctaatc aagcacaaat
      361 aaggggcgtg taacacaaaa gccagcgacc ctttccaatg caaatcaaac ttgcaattct
      421 ttgccgtttt tatcatttaa gtgtcggctt aaggtccact atcagatgta aacagcctta
      481 tctaacaaag gtatcattac attctgaaat tctcaggcat gcaagctaga gtgcttttac
      541 aatgtagatt gtgtcaaagc agcttaactt agaagattag tatagtaaat tgaaactgtg
      601 tcagtccatt tttaagagtt taagttcagt tcagtttagt tcagtgtggg ttaattttta
      661 ctgctgagag tccaaacact aaagagcaaa tccaaataca caagtcaata aataaactca
      721 atgatgggct aaagatctgg ggaaatctac agatttctgt gcgcacagtc agggcctagg
      781 cattacctat actcaacgtt tactctgaga aacttccata ttttagaggc aaaatgctca
      841 gaattgtaaa aagtcttgtc aaata
//
