LOCUS       sequence-66240-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Gradisar H, Bozic S, Doles T, Vengust D, Hafner-Bratkovic I, Mertelj
            A, Webb B, Sali A, Klavzar S, Jerala R
  TITLE     Design of a single-chain polypeptide tetrahedron assembled from 
            coiled-coil segments.
  JOURNAL   Nat Chem Biol. 2013 Apr 28. doi: 10.1038/nchembio.1248.
  PUBMED    23624438
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Apr 28. doi: 10.1038/nchembio.1248."
COMMENT     Sequence Label:  BB-­NIC-­II-­HisN-­TET12
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             38..55
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             464..520
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
ORIGIN
        1 attttgttta actttaagaa ggaggtaaat aatgtatcat caccatcacc atcactctag
       61 agccggcatg aaacagctgg aaaaagagct gaaacagtta gaaaaagaac tgcaagcaat
      121 tgaaaaacag ctggcacagc tgcaatggaa agcacaggca cgtaaaaaaa aactggccca
      181 gctgaaaaaa aaacttcagg cctccggccc aggatccccc gaagatgaga tccagcaact
      241 ggaagaagaa atcgctcagc tggaacagaa aaacgcagcg ctgaaagaga aaaaccaggc
      301 gctgaaatac ggttccggcc caggagatat tgaacaggaa ctggaacgtg caaaagcaag
      361 cattcgtcgt ctggaacaag aagttaatca agaacgtagc cgcatggcat atctgcaaac
      421 cctgctggca aaatcaggtc cgggtcagtt agaagacaaa gttgaagaac tgctgagcaa
      481 gaactaccat ctggaaaatg aagttgcccg tctgaaaaaa ctggttggtt ctggtccggg
      541 tatgaagcaa ttagaaaaag agttaaaaca actggaaaaa gagttacagg ccatcgagaa
      601 acagttagca cagttacagt ggaaagccca ggctcgcaaa aaaaagttag cccaactgaa
      661 gaagaaatta caggcatcag gtcctggtag ccctgaagat gaaatccagg cactggaaga
      721 aaaaaacgcg cagctgaaac aagaaattgc cgcattagag gaaaaaaacc aggcactgaa
      781 atacggttct ggtcctggtc aactggaaga taaggtggaa gaattactgt
//
