LOCUS       sequence-66242-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Yang CF, Chiang MC, Gray DC, Prabhakaran M, Alvarado M, Juntti SA, 
            Unger EK, Wells JA, Shah NM
  TITLE     Sexually dimorphic neurons in the ventromedial hypothalamus govern 
            mating in both sexes and aggression in males.
  JOURNAL   Cell. 2013 May 9;153(4):896-909. doi: 10.1016/j.cell.2013.04.017.
  PUBMED    23663785
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.04"; journalName: "Cell"; date: "2013-05-9- 9";
            volume: "153"; issue: "4"; pages: "896-909"
COMMENT     Sequence Label: pAAV-flex-taCasp3-TEVp
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(40..73)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(124..157)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      166..175
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             694..714
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atccagaggt tgattatcga taagcttgat atcgaattca taacttcgta tagcatacat
       61 tatacgaagt tattctttgc accattctaa agaataacag tgataatttc tgggttaagg
      121 caaataactt cgtataggat actttatacg aagttatgct aggccgccac catggagaac
      181 actgaaaact cagtggattc aaaatccatt aaaaatttgg aaccaaagat catacatgga
      241 agcgaatcaa tggactctgg aatatccctg gacaacagtt ataaaatgga ttatcctgag
      301 atgggtttat gtataataat taataataag aattttcata aaagcactgg aatgacatct
      361 cggtctggta cagatgtcga tgcagcaaac ctcagggaaa cattcagaaa cttgaaatat
      421 gaagtcagga ataaaaatga tcttacacgt gaagaaattg tggaattgat gcgtgatgtt
      481 tctaaagaag atcacagcaa aaggagcagt tttgtttgtg tgcttctgag ccatggtgaa
      541 gaaggaataa tttttggaac aaatggacct gttgacctga aaaaaataac aaactttttc
      601 agaggggatc gttgtagaag tctaactgga aaacccaaac ttttcattat tcaggcctgc
      661 cgtggtacag aactggactg tggcattgag acagaaaatc tctacttcca gagtggtgtt
      721 gatgatgaca tggcgtgtca taaaatacca gtggaggccg acttcttgta tgcatactcc
      781 acagcacctg gttattattc ttggcgaa
//
